Technologies for Single-Cell Isolation
By A. Gross, J. Schoendube, Stefan Zimmermann, Maximilian Steeb, Roland Zengerle, Peter Koltay. International Journal of Molecular Sciences, 2015
383 citations recorded by the source corpus.
The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to identify the most relevant technologies, presented here for the first time, shows that FACS (fluorescence activated cell sorting) respectively Flow cytometry (33% usage), laser microdissection (17%), manual cell picking (17%), random seeding/dilution (15%), and microfluidics/lab-on-a-chip devices (12%) are currently the most frequently used technologies. These most prominent technologies are described in detail and key performance factors are discussed. The survey data indicates a further increasing interest in single-cell isolation tools for the coming years. Additionally, a worldwide patent search was performed to screen for emerging technologies that might become relevant in the future. In total 179 patents were found, out of which 25 were evaluated by screening the title and abstract to be relevant to the field.
Subfields: Biomedical Engineering
View Paper: https://www.semanticscholar.org/paper/58d6eb290ea81211e9e960893391b958729b7671
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Citations in the corpus
- Single-Cell RNA-Seq Technologies and Related Computational Data Analysis (2019, methodology): “Currently, several different approaches are available for isolating single cells, including limiting dilution, micromanipulation, flow-activated cell sorting (FACS), laser capture microdissection (LCM), and microfluidics (Gross et al., 2015; Kolodziejczyk et al., 2015; Hwang et al., 2018).”