The dynamic epitranscriptome: N6-methyladenosine and gene expression control
By Kate D. Meyer, Samie R. Jaffrey. Nature reviews. Molecular cell biology, 2014
959 citations recorded by the source corpus.
N6-methyladenosine (m6A) is a modified base that has long been known to be present in non-coding RNAs, ribosomal RNA, polyadenylated RNA and at least one mammalian mRNA. However, our understanding of the prevalence of this modification has been fundamentally redefined by transcriptome-wide m6A mapping studies, which have shown that m6A is present in a large subset of the transcriptome in specific regions of mRNA. This suggests that mRNA may undergo post-transcriptional methylation to regulate its fate and function, which is analogous to methyl modifications in DNA. Thus, the pattern of methylation constitutes an mRNA 'epitranscriptome'. The identification of adenosine methyltransferases ('writers'), m6A demethylating enzymes ('erasers') and m6A-binding proteins ('readers') is helping to define cellular pathways for the post-transcriptional regulation of mRNAs.
Subfields: Cancer Research, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/d3deb5406f07ce0b2bfe935a1c97fc1f273f9845
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Citations in the corpus
- Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA (2016, background): “In human cells, thousands of mRNAs are subject to m(6)A modification (28, 29), which has been implicated in mRNA splicing, nuclear export, and translation (31) as well as microRNA processing (32).”
- METTL3 promotes translation in human cancer cells (2016, neutral): “…and stem cell self-renewal and differentiation (Batista et al., 2014; Chen et al., 2015; Fustin et al., 2013; Geula et al., 2015; Iles et al., 2013; Lin and Gregory, 2014; Wang et al., 2014c; Zhao et al., 2014; Zheng et al., 2013) (reviewed in Meyer and Jaffrey, 2014; Yue et al., 2015).”
- Rethinking m6A Readers, Writers, and Erasers (2017, methodology): “Early profiling studies reported m6A antibody-based enrichment of RNA fragments mapping near the transcription start site of mRNAs (Meyer & Jaffrey 2014), but due to the low resolution of these techniques, it was difficult to determine whether this enrichment reflected m6A or m6Am residues.”
- Structural basis for cooperative function of Mettl3 and Mettl14 methyltransferases (2016, positive): “Recent studies have intensely investigated howm(6)Amodification of RNA contributes to central events in biology (Fu et al., 2014; Liu and Pan, 2016; Meyer and Jaffrey, 2014).”
- N6-methyladenosine Modulates Messenger RNA Translation Efficiency (2015, positive): “, 2014), and N-methyladenosinde (Fu et al., 2014; Lee et al., 2014; Meyer and Jaffrey, 2014; Nilsen, 2014; Wang and He, 2014a), have emerged as potential new mechanisms of post-transcriptional gene regulation.”
- Temporal Control of Mammalian Cortical Neurogenesis by m6A Methylation (2017, neutral): “…m6A is the most abundant internal modification in mRNAs of eukaryotic cells (Desrosiers et al., 1975). m6A profiling with cell lines has revealed m6A sites in over 25% of human transcripts, with enrichment in long exons, and near transcription start sites and stop codons (Meyer and Jaffrey, 2014).”
- RNA N6-methyladenosine methylation in post-transcriptional gene expression regulation (2015, methodology): “In 2012, two groups independently developed an antibody-based highthroughput sequencing method (Dominissini et al. 2012; Meyer et al. 2012) and for the first time profiled the transcriptome-wide m6A distribution.”