An improved smaller biotin ligase for BioID proximity labeling

By D. I. Kim, Samuel C. Jensen, Kyle A. Noble, Birendra Kc, Kenneth H. Roux, K. Motamedchaboki, Kyle J Roux. Molecular Biology of the Cell, 2016

702 citations recorded by the source corpus.

A smaller promiscuous biotin ligase for proximity biotinylation called BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, exhibits enhanced labeling of proximate proteins, and demonstrates the use of a flexible linker to modulate the biotin-labeling radius.

Subfields: Cell Biology, Organic Chemistry

View Paper: https://www.semanticscholar.org/paper/84e3c21817ec11fa28d1bb2cc8c6d188c0caa7ea

Citations in the corpus

• Efficient proximity labeling in living cells and organisms with TurboID (2018, unknown): “The newer promiscuous ligases BioID2 26 and BASU 27 are also included in the comparison, and after normalization to account for differences in ligase expression levels, give activities comparable to that of BioID (Figure 2a, b).”