Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging

By Hyun-Woo Rhee, P. Zou, N. Udeshi, Jeffrey D. Martell, Vamsi K. Mootha, Steven A. Carr, A. Ting. Science, 2013

1,219 citations recorded by the source corpus.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/347420c8fca6c60ef43db41fd0c0f42804a187da

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Sarah E. Calvo
2. Amit Fulzele
3. Karl R. Clauser
4. Gene W. Yeo
5. M. Larance
6. Vamsi K. Mootha
7. Angus I. Lamond
8. Patrick S. Ward

In Spectroscopy

1. M. Larance
2. Vamsi K. Mootha
3. Angus I. Lamond

Citations in the corpus

• Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules (2018, methodology): “In this study, we use a combination of ascorbate peroxidase (APEX)-mediated in vivo proximity labeling (Rhee et al., 2013) with quantitative mass spectrometry (MS) and an RBP-focused immunofluorescence (IF) approach to comprehensively and significantly expand the repertoire of known SG proteins acro”
• Efficient proximity labeling in living cells and organisms with TurboID (2018, neutral): “…using true positive and false positive protein lists for each organelle (Supplementary Table 2, Supplementary Table 3, and Supplementary Table 4) 2,33 , to obtain BioID/TurboID/miniTurbo-derived proteomes for the ERM (Supplementary Table 5), nucleus (Supplementary Table 6), and mitochondrial…”
• Multidimensional proteomics for cell biology (2015, methodology): “3b) , for example, on the basis of the ectopic expression of a protein of interest fused to either a promiscuous bio-tin-ligase derived from bacteria (the BioID method) 68 or to a peroxidase enzyme capable of activating biotin–phenol (the APEX method) 39 .”
• MitoCarta2.0: an updated inventory of mammalian mitochondrial proteins (2015, background): “We include the APEX-based matrix and IMS proteomes in T mito given the extremely high specificity of APEX-labeling.”
• MitoCarta3.0: an updated mitochondrial proteome now with sub-organelle localization and pathway annotations (2020, methodology): “0 (5) using similar methods on updated protein models and incorporating evidence from proximity proteomics (6,7).”