Grape exosome-like nanoparticles induce intestinal stem cells and protect mice from DSS-induced colitis.

By Songwen Ju, J. Mu, T. Dokland, Xiaoying Zhuang, Qi-long Wang, Hong Jiang, Xiaoyu Xiang, Zhong-bin Deng, Baomei Wang, Lifeng Zhang and 6 more. Molecular Therapy, 2013

782 citations recorded by the source corpus.

Food-derived exosome-like nanoparticles pass through the intestinal tract throughout our lives, but little is known about their impact or function. Here, as a proof of concept, we show that the cells targeted by grape exosome-like nanoparticles (GELNs) are intestinal stem cells whose responses underlie the GELN-mediated intestinal tissue remodeling and protection against dextran sulfate sodium (DSS)-induced colitis. This finding is further supported by the fact that coculturing of crypt or sorted Lgr5⁺ stem cells with GELNs markedly improved organoid formation. GELN lipids play a role in induction of Lgr5⁺ stem cells, and the liposome-like nanoparticles (LLNs) assembled with lipids from GELNs are required for in vivo targeting of intestinal stem cells. Blocking β-catenin-mediated signaling pathways of GELN recipient cells attenuates the production of Lgr5⁺ stem cells. Thus, GELNs not only modulate intestinal tissue renewal processes, but can participate in the remodeling of it in response to pathological triggers.

Subfields: Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/d133a0cc5acdb7f7f851697e2366279f33b7611a

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In Molecular Biology

1. M. Kim
2. E. Batrakova

Citations in the corpus

• Applying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper (2015, neutral): “dextran sulphate sodium-induced colitis (247).”
• Plant exosome like nanovesicles: Emerging therapeutics and drug delivery nanoplatforms. (2020, background): “Lipid profiling of PELNVs has revealed two distinct group of lipids, phospholipids and glycerol lipids, which have been found to be vital for PELNV stability, uptake, and other biological functions.(86) Many PELNVs harbor PA, which modulates membrane fission and fusion, being the dominant lipid medi”
• Using exosomes, naturally-equipped nanocarriers, for drug delivery (2015, background): “showed protective effects of exosome-like nano-particles isolated from crushed grapes [86].”
• Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer. (2016, background): “We further found that the protein content of GDNPs was low, and was primarily in the form of cytosolic proteins, with few membrane proteins, an observation in agreement with the protein profile reported for grape nanoparticles [29].”
• Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles (2014, positive): “The miRNA profile [20] of grape exosome-like nanoparticles was used for predicting Grape EPDEN-miRNA homologue sequences to other species using ClustalX2 analysis http:// www.”