Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles
By J. Mu, Xiaoying Zhuang, Qi-long Wang, Hong Jiang, Zhong-bin Deng, Baomei Wang, Lifeng Zhang, S. Kakar, Yan Jun, Donald R. Miller and 1 more. Molecular Nutrition & Food Research, 2014
658 citations recorded by the source corpus.
Scope Exosomes, small vesicles participating in intercellular communication have been extensively studied recently; however, the role of edible plant derived exosomes in interspecies communication has not been investigated. Here, we investigate the biological effects of edible plant derived exosome-like nanoparticles (EPDEN) on mammalian cells. Methods and results In this study, exosome-like nanoparticles from four edible plants were isolated and characterized. We show that these EPDENs contain proteins, lipids and microRNA. EPDENs are taken up by intestinal macrophages and stem cells. The results generated from EPDEN transfected macrophages indicate that ginger EPDENs preferentially induce the expression of the anti-oxidation gene, heme oxygenase-1 (HO-1) and the anti-inflammatory cytokine, IL-10; whereas grapefruit, ginger, and carrot EPDENs promote activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2). Furthermore, analysis of the intestines of canonical Wnt reporter mice, i.e., B6.Cg-Tg(BAT-lacZ)3Picc/J mice, revealed that the numbers of β-galactosidase+ (β-Gal) intestinal crypts are increased, suggesting that EPDEN treatment of mice leads to Wnt mediated activation of the Tcf4 transcription machinery in the crypts. Conclusion The data suggest a role for EPDEN mediated interspecies communication by inducing expression of genes for anti-inflammation cytokines, anti-oxidation and activation of Wnt signaling, which are crucial for maintaining intestinal homeostasis.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/63ec9bed83fcda0444925a4715335c2c7877d15c
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
Citations in the corpus
- Plant-derived exosomal microRNAs shape the gut microbiota (2018, background): “GELN-RNA Shapes Gut Microbiota A number of edible nanoparticles have been characterized based on electron microscopy (EM) examination (Mu et al., 2014).”
- Plant exosome like nanovesicles: Emerging therapeutics and drug delivery nanoplatforms. (2020, methodology): “As an illustration, multifarious ELNVs derived from grapefruit, ginger, carrots, and tomatoes have been reported.(65) In addition, atomic force microscopy (AFM) can also be used to develop the structural and size-related understanding of individual PELNVs.”
- Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer. (2016, methodology): “Recently, exosome-like nanoparticles isolated from edible plants using an eco-friendly protocol have been characterized [28].”
- Naturally Occurring Exosome Vesicles as Potential Delivery Vehicle for Bioactive Compounds (2019, background): “Changes in the size and surface charge of exosomes depend on the plant source and environment (Mu et al., 2014).”