Serum MicroRNAs Are Promising Novel Biomarkers
By S. Gilad, E. Meiri, Y. Yogev, S. Benjamin, D. Lebanony, N. Yerushalmi, H. Benjamin, M. Kushnir, Hila Cholakh, N. Melamed and 4 more. PLoS ONE, 2008
1,392 citations recorded by the source corpus.
Background Circulating nucleic acids (CNAs) offer unique opportunities for early diagnosis of clinical conditions. Here we show that microRNAs, a family of small non-coding regulatory RNAs involved in human development and pathology, are present in bodily fluids and represent new effective biomarkers. Methods and Results After developing protocols for extracting and quantifying microRNAs in serum and other body fluids, the serum microRNA profiles of several healthy individuals were determined and found to be similar, validating the robustness of our methods. To address the possibility that the abundance of specific microRNAs might change during physiological or pathological conditions, serum microRNA levels in pregnant and non pregnant women were compared. In sera from pregnant women, microRNAs associated with human placenta were significantly elevated and their levels correlated with pregnancy stage. Conclusions and Significance Considering the central role of microRNAs in development and disease, our results highlight the medically relevant potential of determining microRNA levels in serum and other body fluids. Thus, microRNAs are a new class of CNAs that promise to serve as useful clinical biomarkers.
Subfields: Cancer Research, Immunology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/d12ee1ab3abcc59cb1d7701cba50f9b96eac48c1
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Citations in the corpus
- Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. (2012, background): “[61] compared serum miRNA levels in pregnant and non-pregnant women.”
- Detection of microRNA Expression in Human Peripheral Blood Microvesicles (2008, background): “While it has been reported that miRNAs are detected in the plasma [41,42,61–63], this is the first study to profile miRNAs from the plasma microvesicles in a normal healthy population.”
- Secreted monocytic miR-150 enhances targeted endothelial cell migration. (2010, background): “and two other groups reported that circulating miRNAs are promising biomarkers for prostate cancer, pregnancy, and ovarian cancer (Mitchell et al., 2008; Gilad et al., 2008; Resnick et al., 2009).”
- Circulating microRNAs as Novel Minimally Invasive Biomarkers for Breast Cancer (2010, background): “Previous studies, although few in number, have reported discrepancies between serum and plasma miRNA expression levels, or the investigators have chosen either medium alone for use in their studies.(12,13,16) Finally, a potential relationship between circulating miRNA levels and existing clinicopath”