A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback
By Diethard Tautz, C. Pfeifle. 1989
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Citations in the corpus
- Systematic determination of patterns of gene expression during Drosophila embryogenesis (2002, methodology): “RNA probes were hybridized to fixed Drosophila embryos [19] in 96-well plates (see Materials and methods).”
- An introduction to spatial transcriptomics for biomedical research (2022, methodology): “This in situ hybridization (ISH) was first used on a whole organism (Drosophila) in 1989 [17, 42].”
- A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors (2005, methodology): “Transcripts were detected by whole-mount in situ hybridization using digoxigenin-labeled antisense mRNA and visualized by the alkaline phosphatase-NBT/BCIP reaction ( Roche”
- Third-generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust (2018, background): “…thick tissue sections, autofluo-rescence within the sample necessitates the use of in situ amplification to boost the signal-to-background ratio (Tautz & Pfeifle, 1989; Harland, 1991; Lehmann & Tautz, 1994; Kerstens et al., 1995; Nieto et al., 1996; Wiedorn et al., 1999; Player et al., 2001;…”
- Determination of gene expression patterns using high-throughput RNA in situ hybridization to whole-mount Drosophila embryos (2009, background): “A major technical advance in the late 1980s by Tautz and Pfeifl”
- Museum of spatial transcriptomics (2020, unknown): “References: 1994 WMISH [17], 1995 mouse WMISH [18], 1998 AXelDB [19], 1999 GXD [20], 2000 MAGEST [21], 2001 NEXTDB [22], 2001 GHOST [23], 2002 GenePaint [24], 2002 BDGP [25], 2003 MEPD [26], 2003 ZFIN [27], 2004 GEISHA [28], 2005 miRNA [29], 2006 Allen [30], 2006 BDTNP [31], 2007 Fly-FISH [32], 2007”
- Targeted gene expression in Drosophila dopaminergic cells using regulatory sequences from tyrosine hydroxylase. (2003, methodology): “Whole-mount embryo RNA in situ hybridization was performed essentially as described by Tautz and Pfeifle (1989) .”