Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast

By P. Zegerman, J. Diffley. 2007

545 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology

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Citations in the corpus

In silico protein interaction screening uncovers DONSON’s role in replication initiation (2023, background): “Disordered DONSON residues (1-6, 26-154, 330-369) were deleted, leaving only the globular domain and GINS-binding peptide (residues 7-25).”
Phosphorylation-dependent assembly of DNA damage response systems and the central roles of TOPBP1 (2021, background): “Comparison of these with biochemically identified binding motifs for Rad4/Dpb11 from S.pombe Rad9 and S.cerevisiae Sld2 (Fig.”
DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in C. elegans (2023, unknown): “Meanwhile, CDK phosphorylates both Sld3 and Sld2 (Figure S1, step 3) at sites that are recognized by pairs of BRCT domains in the amino-terminus (binds phospho-Sld3) and carboxyl-terminus (binds phospho-Sld2) of Dpb11 (18, 19).”
Concerted Loading of Mcm2-7 Double Hexamers Around DNA during DNA Replication Origin Licensing (2009, background): “CDKs work by phosphorylating Sld2 and Sld3, which generates binding sites for tandem pairs of BRCT repeats in Dpb11 (Tanaka et al., 2007; Zegerman and Diffley, 2007), while DDK phosphorylates Mcm2–7 directly (Sheu and Stillman, 2006).”
Efficient Initiation of DNA Replication in Eukaryotes Requires Dpb11/TopBP1-GINS Interaction (2013, methodology): “One of the approaches used to induce CDK bypass replication is the combination of phosphomimetic Sld2 and the “SD fusion” protein, in which BRCT1 and -2 of Dpb11 are replaced with an unphosphorylatable Sld3, Sld3-2A (5).”
A vertebrate gene, ticrr, is an essential checkpoint and replication regulator. (2010, background): “In particular, we wondered whether Ticrr might be the vertebrate ortholog of the budding yeast protein Sld3, which interacts with Dpb11 and is required for DNA replication initiation (Kamimura et al. 2001; Tanaka et al. 2007; Zegerman and Diffley 2007).”
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7 (2020, background): “Current models propose that a network of interactions between Sld3, Dpb11, Sld2, and GINS are required to form the CMG (Tanaka et al., 2007; Zegerman and Diffley, 2007; Muramatsu et al., 2010).”
CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast. (2010, background): “…replication origins because Sld3, together with Cdc45, associates with origins before and independently of the pre-LC (Kamimura et al. 2001), and because phosphorylated 608 GENES & DEVELOPMENT Sld3 binds to the N-terminal tandem BRCT domain of Dpb11 (Tanaka et al. 2007; Zegerman and Diffley 2007).”
Treslin Collaborates with TopBP1 in Triggering the Initiation of DNA Replication (2010, background): “Hence, Dpb11 appears to be acting, at least in part, as a scaffolding protein that helps to position other replication proteins for initiation.”
MTBP, the partner of Treslin, contains a novel DNA-binding domain that is essential for proper initiation of DNA replication (2017, background): “Studies in the budding yeast system established that S-Cdk phosphorylates proteins known as Sld2 and Sld3 (Tanaka et al., 2007; Zegerman and Diffley, 2007; Labib, 2010; Siddiqui et al., 2013; Tanaka and Araki, 2013).”