Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

By C. Brangwynne, T. Mitchison, Anthony A. Hyman. Proceedings of the National Academy of Sciences of the United States of America, 2011

1,159 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4f24c0afe38d98561491a84e0d12cfcc01620080

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Citations in the corpus

Compositional Control of Phase-Separated Cellular Bodies (2016, positive): “Recent work has demonstrated that bodies exhibit liquid-like properties (Brangwynne et al., 2009; Brangwynne et al., 2011; Chen et al., 2008; Kroschwald et al., 2015; Patel et al., 2015; Wang et al., 2014).”
Principles and Properties of Stress granules (2016, background): “For example, nucleoli require ATP for their own liquidlike behavior, and are 10 –fold less dynamic when ATP is depleted [23].”
RNA controls PolyQ protein phase transitions (2015, background): “As we have previously described (Brangwynne et al., 2011), we plot the change in aspect ratio l/w, where l is the long axis and w is the short axis of fusing droplets, over time.”
Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein (2016, background): “…2009; Elbaum-Garfinkle et al., 72 Molecular Cell 63, 72–85, July 7, 2016 ª 2016 Elsevier Inc. 2015; Wang et al., 2014), nucleoli of Xenopus oocytes (Brangwynne et al., 2011) and C. elegans embryos (Berry et al., 2015), stress granules in mammalian cells (Kroschwald et al., 2015), and…”
A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation. (2015, positive): “Recently, an increasing number of these non-membrane-bound compartments have been shown to behave like condensed liquid phases of the cytoplasm or nucleoplasm (Aggarwal et al., 2013; Brangwynne et al., 2009, 2011; Hubstenberger et al., 2013; Lee et al., 2013; Wippich et al., 2013).”
Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles (2015, background): “Moreover, spherical nucleoli of the amphibian oocyte have been observed to coalesce when in close contact and show a size distribution that obeys a simple power law, indicating the formation of liquid droplets (Brangwynne et al., 2011).”
Formation and Maturation of Phase Separated Liquid Droplets by RNA Binding Proteins (2015, background): “…has suggested that P granules and the nucleolus behave as phase-separated liquids, being round in shape, undergoing cycles of fusion and fission, and distorting in response to shear forces (Brangwynne et al., 2009, 2011; Elbaum-Garfinkle et al., 2015; Weber and Brangwynne, 2015; Wang et al., 2014).”
Liquid-liquid phase separation in biology. (2014, background): “A further example of a liquid-like compartment is the nucleolus of Xenopus germinal vesicles (Brangwynne et al. 2011).”
Phase separation in biology; functional organization of a higher order (2016, methodology): “Microrheology methods, traditionally used in the characterization of viscoelastic properties of polymers and complex fluids [104], were applied to the characterization of membrane-less organelles [5, 6, 42, 105] and in vitro formed protein and protein-RNA liquid droplets [47, 55].”