DNA methylation on N6-adenine in C. elegans

By E. L. Greer, M. A. Blanco, Lei Gu, Erdem Sendinc, Jianzhao Liu, David Aristizábal-Corrales, Chih-Hung Hsu, L. Aravind, Chuan He, Yang Shi. Cell, 2015

620 citations recorded by the source corpus.

Summary In mammalian cells, DNA methylation on the 5th position of cytosine (5mC) plays an important role as an epigenetic mark. However, DNA methylation was considered to be absent in C. elegans because of the lack of detectable 5mC as well as homologs of the cytosine DNA methyltransferases. Here, using multiple approaches, we demonstrate the presence of adenine N6-methylation (6mA) in C. elegans DNA. We further demonstrate that this modification increases trans-generationally in a paradigm of epigenetic inheritance. Importantly, we identify a DNA demethylase, NMAD-1, and a potential DNA methyltransferase, DAMT-1, which regulate 6mA levels and crosstalk between methylation of histone H3K4me2 and 6mA, and control the epigenetic inheritance of phenotypes associated with the loss of the H3K4me2 demethylase spr-5. Together, these data identify a DNA modification in C. elegans and raise the exciting possibility that 6mA may be a carrier of heritable epigenetic information in eukaryotes.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c9cdfe0abf89699ecbf1d5467d9a2a314262fcaf

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