Substrate compliance versus ligand density in cell on gel responses.

By A. Engler, L. Bačáková, C. Newman, A. Hategan, Maureen A. Griffin, Dennis E. Discher. Biophysical Journal, 2004

1,144 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Cell Biology, Immunology and Allergy

View Paper: https://www.semanticscholar.org/paper/c8c264035eab102452c4157ad5144454c7d1ac04

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Citations in the corpus

Extracellular matrix rigidity governs smooth muscle cell motility in a biphasic fashion (2005, methodology): “Taken together, these results definitively show that focal adhesion and actin cytoskeletal assembly in SMCs depend on the mechanical properties of the underlying ECM, as previously suggested (Pelham and Wang, 1998; Engler et al., 2004).”
Physics of adherent cells (2013, methodology): “In particular, it was found that skeletal muscle cells differentiate optimally on substrates with rigidities of 11 kPa (Engler et al., 2004b) and that stem-cell fate strongly depends on substrate rigidity (Engler et al., 2006).”
The effect of substrate stiffness on adult neural stem cell behavior. (2009, background): “Recent work has shown that the mechanical properties of the matrix impact the differentiation profile of stem cells [10].”
Matrices with compliance comparable to that of brain tissue select neuronal over glial growth in mixed cortical cultures. (2006, background): “On soft gels astrocytes show fewer stress fibers, consistent with previous findings in fibroblasts where stress fibers fail to form (11).”
Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating (2008, methodology): “The observation that matricellular composition and mechanics can direct cell function is not specific to heart (see Engler et al., 2004b; Paszek et al., 2005; Yeung et al., 2005).”
Matrix Stiffness Modulates Proliferation, Chemotherapeutic Response and Dormancy in Hepatocellular Carcinoma Cells (2011, methodology): “In this system, matrix stiffness is altered by modulating the bis-acrylamide crosslink density of thin PA gels without altering the surface composition or density of ligands to which the cells are exposed (13).”