Screening methods to determine antibacterial activity of natural products
By Cleidson Valgas, S. M. Souza, Elza F. A. Smânia, A. Smânia. 2007
1,360 citations recorded by the source corpus.
The emergence of new infectious diseases, the resurgence of several infections that appeared to have been controlled and the increase in bacterial resistance have created the necessity for studies directed towards the development of new antimicrobials. Considering the failure to acquire new molecules with antimicrobial properties from microorganisms, the optimization for screening methods used for the identification of antimicrobials from other natural sources is of great importance. The objective of this study was to evaluate technical variants used in screening methods to determine antibacterial activity of natural products. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, by two variants of the agar diffusion method (well and disc), two variants of the bioautographic method (direct and indirect) and by microdilution assay. We concluded that the well-variant of the diffusion method was more sensitive than the disc-variant, whilst the direct-variant of the bioautographic method exhibited a greater sensitivity if compared to indirect-variant. Bioautographic and diffusion techniques were found to have similar sensitivity; however the latter technique provided more suitable conditions for the microbial growth. In this study, we also discussed the best conditions for the determination of minimal inhibitory concentration.
Subfields: Cell Biology, Ecology, Evolution, Behavior and Systematics, Food Science
View Paper: https://www.semanticscholar.org/paper/c7bded6fb0bcea47b1939baf274f99ef80f99708
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In Ecology, Evolution, Behavior and Systematics
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Citations in the corpus
- Tetrazolium/Formazan Test as an Efficient Method to Determine Fungal Chitosan Antimicrobial Activity (2013, methodology): “The agar diffusionmethod is the most frequently used [10, 11] and has been standardized as an official test method for detecting”
- Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. (2010, methodology): “…activity and since not all of them are based on the same principles, the results obtained are influenced not only by the method selected, but also by the microorganisms used, and by the extraction method or the degree of solubility of each test-compound (Valgas et al., 2007; Tripoli et al., 2007).”
- Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis mellifera (2013, background): “The antimicrobial effectiveness reported have been evaluated with diverse sets of methodologies, degrees of sensitivity and microbial strains, what leads to difficulties comparing results from work teams (Vargas et al., 2007).”