Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization

By A. Yildiz, J. Forkey, S. McKinney, T. Ha, Y. Goldman, P. Selvin. Science, 2003

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Molecular motor traffic: From biological nanomachines to macroscopic transport (2006, background): “to ‘hand-over-hand’ motion [ 5 ] but with a step size of about 36 nm for the center-of-mass displacement [36] which corresponds to a displacement of about 72 nm for a single head.”
Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function (2009, background): “Because single-molecule emitters are only a few nanometers in size, they represent particularly useful point sources for imaging, and superlocalization of single molecules at room temperature has been pushed to the 1-nm regime (9) in transverse (2-dimensional) imaging.”
Photophysics of Fluorescence Probes for Single Molecule Biophysics and Super-Resolution Imaging (2012, positive): “5 nm accuracy(17) and most recently to a sub-nm level(18).”
Super resolution fluorescence microscopy (2009, background): “Taking advantage of single-molecule detection and imaging (34,35), nanometer localization precision has also been achieved for single fluorescent molecules under ambient conditions (36).”
A molecular ruler based on plasmon coupling of single gold and silver nanoparticles (2005, background): “dtd" [ ]> Forster Resonance Energy Transfer has served as a molecular ruler that reports conformational changes and intramolecular distances of single biomolecule”