Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function
By S. R. P. Pavani, M. A. Thompson, J. Biteen, Samuel J Lord, Na Liu, R. Twieg, R. Piestun, W. Moerner. Proceedings of the National Academy of Sciences of the United States of America, 2009
789 citations recorded by the source corpus.
Subfields: Biomedical Engineering, Biophysics, Electrical and Electronic Engineering
View Paper: https://www.semanticscholar.org/paper/7d17746f81c6c47b9ad840ed9bee574d466f45de
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In Biomedical Engineering
In Biophysics
Citations in the corpus
- Inaugural Article: Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function (2010, positive): “More details on the calibration of the DH-PSF imaging system can be found in previous work (27, 59).”
- Fluorophore localization algorithms for super-resolution microscopy (2014, methodology): “Estimating z involves comparing the widths of two different images, which is feasible via LS fits to measured (rather than theoretical) PSFs, yielding results that compare favorably with astigmatism 42 .”
- A guide to super-resolution fluorescence microscopy (2010, methodology): “…over an extended imaging depth of a few microns by either introducing an astigmatism, as in 3D STORM (Huang et al., 2008a,b), by a double-plane detection in biplane (BP) FPALM (Juette et al., 2008), or by engineering a double helix–shaped point spread function (DH-PSF; Pavani et al., 2009).”