Identifying wood-inhabiting fungi with 454 sequencing – what is the probability that BLAST gives the correct species?

By Otso Ovaskainen, Jussi Nokso-Koivisto, J. Hottola, T. Rajala, T. Pennanen, Heini Ali-Kovero, O. Miettinen, Petri Oinonen, P. Auvinen, L. Paulin and 2 more. 2010

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Using metabarcoding to ask if easily collected soil and leaf-litter samples can be used as a general biodiversity indicator (2014, background): “…t 2 2 p T c f a t 80 C. Yang et al. / Ecological alvignac-Spencer et al., 2013; Fonseca et al., 2010; Hajibabaei t al., 2012; Hiiesalu et al., 2012; Ovaskainen et al., 2010; Thomsen t al., 2012; Yoccoz et al., 2012; Yu et al., 2012) and can recover cological information in the form of alpha- and…”
Endophytic fungal diversity: review of traditional and molecular techniques (2012, background): “…in natural environments, such as clinical fungi (Gharizadeh et al. 2004), phyllosphere fungi (Jumpponen and Jones 2009), wood-inhabiting fungi (Ovaskainen et al. 2010), soil fungi (Jumpponen et al. 2010), freshwater fungi (Monchy et al. 2011), and mycorrhizal fungi (Lumini et al. 2010;…”
Five simple guidelines for establishing basic authenticity and reliability of newly generated fungal ITS sequences. (2012, background): “Ross et al. (2008) and Ovaskainen et al. (2010) provide interesting statistics on the performance of BLAST under varying conditions, including incomplete database coverage.”