Endophytic fungal diversity: review of traditional and molecular techniques
By Xiang Sun, Liang-Dong Guo. Mycology, 2012
165 citations recorded by the source corpus.
Endophytic fungi are an important component, are ubiquitous and occur within all know plants, including a broad range of hosts in various ecosystems, and therefore play an important role in the natural environment. More than 1 million species of endophytic fungi are estimated to exist based on a ratio of vascular plants to fungal species of 1:4 or 1:5. Nevertheless, our recognition of endophyte diversity is limited at present. In surveys of endophyte diversity, traditional techniques, such as cultivation-dependent methods, have been routinely used in previous studies. The discovery of endophytic fungi in natural environments, however, has been limited by traditional methodology due to some non-sporulating and non-culturable fungi. Molecular techniques, such as DNA fingerprinting and sequencing methods, have been successfully employed in the detection and identification of endophytic fungi, and different endophyte diversity and community composition have been documented by cultivation-dependent and molecular techniques. This review paper summarises recent progress in the study of endophytic fungal diversity and some key questions are highlighted for future research in endophyte biology.
Subfields: Cell Biology, Ecology, Evolution, Behavior and Systematics, Plant Science
View Paper: https://www.semanticscholar.org/paper/d970354449037fa31da9ce6546777cfb5c598e01
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Citations in the corpus
- Induction of sporulation in plant pathogenic fungi (2012, background): “Sterile mycelia are often encountered in taxonomic and ecological studies (Suryanarayanan and Vijaykrishna 2001; Su et al. 2010; Tejesvi et al. 2011; Sun and Guo 2012).”
- Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation (2020, methodology): “It is important to provide suitable conditions to yield optimal growth, the viability of mycelia however, depends on the growth rate, culture media and method of storage (Sun & Guo 2012).”