ER Tubules Mark Sites of Mitochondrial Division

By Jonathan R. Friedman, L. Lackner, Matthew West, Jared R. DiBenedetto, J. Nunnari, G. Voeltz. Science, 2011

1,996 citations recorded by the source corpus.

Mitochondrial division occurs at positions where endoplasmic reticulum tubules contact mitochondria and mediate constriction. Mitochondrial structure and distribution are regulated by division and fusion events. Mitochondrial division is regulated by Dnm1/Drp1, a dynamin-related protein that forms helices around mitochondria to mediate fission. Little is known about what determines sites of mitochondrial fission within the mitochondrial network. The endoplasmic reticulum (ER) and mitochondria exhibit tightly coupled dynamics and have extensive contacts. We tested whether ER plays a role in mitochondrial division. We found that mitochondrial division occurred at positions where ER tubules contacted mitochondria and mediated constriction before Drp1 recruitment. Thus, ER tubules may play an active role in defining the position of mitochondrial division sites.

Subfields: Clinical Biochemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/c529053c8d1ea3f000124e8a244d44a8c86e0859

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Citations in the corpus

Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics (2015, background): “5A, bottom) and movies (Movie 10) of Skylan-NS–tagged translocase of outer mitochondrial membrane 20 (TOM20) revealed the migration, constriction before fission, and fusion of individual mitochondria (43, 44), each clearly resolved as a hollow tubular structure.”
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Mitophagy: mechanisms, pathophysiological roles, and analysis (2012, background): “It is also possible that the paternal mitochondria in C. elegans may use other tags for selective removal through autophagy in addition to ubiquitin.”
Mitochondria: In Sickness and in Health (2012, background): “We provide a current view of how mitochondrial functions impinge on health and disease.”
Mitochondrial dynamics regulates migration and invasion of breast cancer cells (2012, background): “Thus, this meshwork of mitochondria needs to be sectioned via fission as it is being repositioned (13).”
Mitochondria-lysosome contacts regulate mitochondrial fission via Rab7 GTP hydrolysis (2018, neutral): “…Walther Mothes (Addgene #1817) 32 , BFP-KDEL, mito-BFP, mCh-Drp1 and mCh-Rab7A were gifts from Gia Voeltz (Addgene #49150, #49151, #49152, #61804) 10,33 , EGFP-LC3 was a gift from Karla Kirkegaard (Addgene #11546) 34 , pMXs-puro GFP-DFCP1 was a gift from Noboru Mizushima (Addgene #38269) 35 ,…”
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