Purification and Characterization of PCR-Inhibitory Components in Blood Cells

By W. Al-Soud, P. Rådström. Journal of Clinical Microbiology, 2001

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Diagnosis of Lyme Borreliosis (2005, background): “The low yield could be a reflection of lack of spirochetemia or transient spirochetemia (22), a low level of spirochetes in blood (108), and/or the presence of PCR inhibitors in host blood (2, 62, 345).”
Advances in Microfluidic PCR for Point-of-Care Infectious Disease Diagnostics (2011, background): “In addition, the large human genomic content from the blood cells contributes to the PCR background and can significantly compromise the amplification efficiency of the target sequence (Al-Soud and Radstrom, 2001).”
Analytical Methods for Virus Detection in Water and Food (2011, background): “Several ways have been described to overcome this inhibition, such as the analysis of samples dilutions, smaller sample sizes, adaptation of the PCR by, e.g., the addition of Tween, BSA, or commercial reagents (Rutjes et al. 2005; Butot et al. 2007; Al-Soud and Radstrom 2001).”
PCR inhibitors – occurrence, properties and removal (2012, methodology): “Generally, the effects of the inhibitors may be reduced by selecting an appropriate method for sample processing and nucleic acid extraction, by the choice of a more robust DNA polymerase or by the use of specific PCR additives (Al-Soud and Rådström 2001).”