Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry

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A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language (2011, background): “Adjusting the solvent composition (organic phase content, basic or acidic pH, buffer concentration) and ionization conditions (polarity mode, declustering energy, interface temperature, etc.) enhances the detection sensitivity by several orders of magnitude [8,9].”
LipidXplorer: A Software for Consensual Cross-Platform Lipidomics (2012, neutral): “identify their molecular species [5,29,31,32] (Figure 1).”
Revitalizing membrane rafts: new tools and insights (2010, background): “Thus, the yeast raft lipids, which are the cholesterol homologue ergosterol and three classes of sphingolipids (inositolphosphoceramide, mannosyl-inositolphosphoceramide and mannosyl-diinositolphosphoceramide”
Lipidomics: coming to grips with lipid diversity (2010, background): “First, progress in tandem mass spectrometry ( Box 2 ) has enabled high-throughput quantitative profiling of the lipidome from minute amounts of samples and the identification of lipids as individual molecular specie”
Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity (2018, methodology): “Lipids were extracted using a two-step chloroform/methanol procedure (Ejsing et al., 2009).”