Generation of transgenic citrus plants with the tolerance-to-salinity gene HAL2 from yeast

By M. Cervera, C. Ortega, A. Navarro, L. Navarro, Leandro Peña. 2000

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Agrobacterium-mediated transformation of embryogenic calluses of Ponkan mandarin and the regeneration of plants containing the chimeric ribonuclease gene (2002, methodology): “The use of epicotyl segments and protoplasts as explants for genetic transformation has been applied widely in most reports using Agrobacterium and electroporation, respectively (Cervera et al. 2000; Olivares-Fuster et al. 2000; Pena and Navarro 1999; Serres and Stang 1992).”
An evaluation of factors affecting the efficiency of Agrobacterium-mediated transformation of Citrus paradisi (Macf.) and production of transgenic plants containing carotenoid biosynthetic genes (2002, background): “…for a number of citrus species and relatives (Kaneyoshi et al. 1994; Peña et al. 1995a, b, 1997, 2001; Gutiérrez-E et al. 1997; Bond and Roose 1998; Cervera et al. 1998, 2000; PérezMolphe-Balch and Ochoa-Alejo 1998; Luth and Moore 1999; Domínguez et al. 2000; Yang et al. 2000; Ghorbel et al. 2001).”
Mutation induction and tissue culture in improving fruits (2001, background): “A gene inducing tolerance to salt stress has been introduced in Carrizo citrange (Citrus sinensis L. Osbeck × Poncirus trifoliata L. Raf) (Cervera et al., 2000).”