Agrobacterium-mediated transformation of embryogenic calluses of Ponkan mandarin and the regeneration of plants containing the chimeric ribonuclease gene

By L. D., S. W., Deng X.. Plant Cell Reports, 2002

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Induction of Seedlessness in Citrus: From Classical Techniques to Emerging Biotechnological Approaches (2008, background): “Li et al. (2002) reported on a transgenic ‘Ponkan’ mandarin transformed with the barnase suicide gene under the control of a tapetum-specific promoter (pTA29).”
Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus (2009, methodology): “These methods include Agrobacterium mediated transformation using juvenile in vitro epicotyl segments (Moore et al. 1992; Luth and Moore 1999 or Ananthakrishnan et al. 2007), mature internode segments obtained from greenhouse-grown plants (Cervera et al. 1998; Almeida et al. 2003a) or embryogenic ca”