Enterobacteriaceae act in concert with the gut microbiota to induce spontaneous and maternally transmitted colitis

By W. Garrett, C. Gallini, Tanya Yatsunenko, M. Michaud, A. Dubois, M. Delaney, Shivesh Punit, M. Karlsson, L. Bry, J. Glickman and 3 more. Cell Host and Microbe, 2010

784 citations recorded by the source corpus.

SUMMARY In inflammatory bowel disease, the relationship between a host and gut microbial community goes awry. We have characterized the fecal microbial communities in a mouse IBD model driven by T-bet deficiency in the innate immune system. 16S rRNA-based analysis of T-bet−/− × Rag2−/− and Rag2−/− mice revealed distinctive communities that correlate with host genotype. Culture-based surveys, invasion assays, antibiotic treatment, and TNF-α blockade disclosed that the presence of Klebsiella pneumoniae and Proteus mirabilis correlates with colitis in T-bet−/− × Rag2−/− animals, and that T-bet−/− × Rag2−/− derived strains can elicit colitis in Rag2−/− and wild-type adults. Cross-fostering experiments provided evidence for the role of these organisms in maternal transmission of disease. This model provides a foundation for defining how gut microbial communities work in concert with specific culturable colitogenic agents to cause IBD, and a foundation for conducting proof-of-concept tests of new preventative or therapeutic measures directed at components of the gut microbiota and/or host.

Subfields: Gastroenterology, Infectious Diseases, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/bbac4d6fc90e5d471532d5f9059846f53a96a678

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Citations in the corpus

A Low-Abundance Biofilm Species Orchestrates Inflammatory Periodontal Disease through the Commensal Microbiota and the Complement Pathway (2011, background): “Enteric pathogens have also been shown to exert global changes on the gut microbiota in rodent models of inflammatory disease (Garrett et al., 2010; Lupp et al., 2007; Stecher et al., 2007).”
Intestinal homeostasis and its breakdown in inflammatory bowel disease (2011, background): “The second study examined a model of transmissible ulcerative-colitis-like disease that arises in T-bet −/− Rag2 −/− (also known as Tbx21 −/− Rag2 −/− ) mice and is associated with defective colonic barrier function and hyperactivation of inflammatory dendritic cell”
Gut microbiota: Role in pathogen colonization, immune responses, and inflammatory disease (2017, background): “Consistently, the relative luminal abundance of Enterobacteriaceae dramatically increased in either chemical -induced or genetic mouse models of IBD (135, 136, 142).”
The composition of the gut microbiota shapes the colon mucus barrier (2014, positive): “The class Deltaproteobacteria contains sulphur-reducing bacteria (SRB) that are also found at higher frequencies in patients with IBD as compared to healthy individuals [41].”
Metagenomic biomarker discovery and explanation (2011, methodology): “We thus sought to investigate the characteristics of the fecal microbiota in a mouse model of spontaneous colitis that occurs in a colony of Balb/c T-bet × Rag2 mice using 16S rRNA gene metagenomic data [66,67].”
Geography, Ethnicity or Subsistence-Specific Variations in Human Microbiome Composition and Diversity (2017, background): “Between-group differences in susceptibilities to many health conditions from preterm birth to type 2 diabetes, obesity and even cancer are being linked to microbiome diversity (Peek and Blaser, 2002; Ley et al., 2005; Turnbaugh et al., 2006; Blaser et al., 2008; Gao et al., 2008; Islami and Kamangar”
Intestinal microbiota in human health and disease: the impact of probiotics (2011, background): “Recently, the presence of two species belonging to the family Enterobacteriaceae, Klebsiella pneumoniae and Proteus mirabilis, was correlated with the development of colitis in a mouse model (Garrett et al. 2010).”
The role of gut microbiota in immune homeostasis and autoimmunity (2012, positive): “A later study discovered that the presence of Klebsiella pneumoniae and Proteus mirabilis in TRUC mice can elicit colitis in SPF but not GF wildtype mice.(71) This suggested that Klebsiella pneumoniae and Proteus mirabilis worked in concert with other members of the endogenous microbial community to”