Expansion of Human Adult Stem Cells from Bone Marrow Stroma: Conditions that Maximize the Yields of Early Progenitors and Evaluate Their Quality

By I. Sekiya, B. Larson, Jason R. Smith, R. Pochampally, J. Cui, D. Prockop. Stem Cells, 2002

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Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle (2007, background): “In addition, nucleated cells were plated at a density such that interactions between cell colonies could be avoided, thereby allowing a maximum rate of expansion (Colter et al. 2000, 2001; Sekiya et al. 2002a; Prockop et al. 2003).”
Aging of mesenchymal stem cells. (2006, background): “Isolation is usually conducted by density centrifugation (sometimes enhanced by gradient solutions) to obtain the mononuclear fraction of marrow cells and by using the widely reported ability of MSC to adhere to tissue culture plastic (Sekiya et al., 2002).”
Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy (2004, background): “high plating densities) [27, 50], and cell surface marker selection [22, 47, 51].”