Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study.

By C. Rotsch, M. Radmacher. Biophysical Journal, 2000

924 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Cell Biology, Physical and Theoretical Chemistry

View Paper: https://www.semanticscholar.org/paper/bb5fc30820ac2ea310630b19ff3dff4f185e2943

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In Atomic and Molecular Physics, and Optics

In Cell Biology

In Physical and Theoretical Chemistry

Citations in the corpus

Cell Stiffness Is a Biomarker of the Metastatic Potential of Ovarian Cancer Cells (2012, background): “Since the actin cytoskeleton contributes to the mechanical properties of the cells, observed variations are consistent with differences in cell stiffness and with previous reports [8,19].”
Substrate compliance versus ligand density in cell on gel responses. (2004, methodology): “The elastic modulus, E, was obtained using a Hertz cone model (Domke and Radmacher, 1998; Rotsch et al., 1999; Rotsch and Radmacher, 2000) to fit the first 5–50 nm of indentation profiles obtained from fully hydrated 1–2-mm gel samples with unsharpened, pyramid-tipped cantilevers of spring constant…”
The optical stretcher: a novel laser tool to micromanipulate cells. (2001, background): “Both microtubules and intermediate filaments are thought to be less important for the elastic strength and structural response of cells subjected to external stress (Petersen et al., 1982; Pasternak and Elson, 1985; Heidemann et al., 1999; Rotsch and Radmacher, 2000).”
The biophysics of neuronal growth (2010, background): “These properties are largely governed by the cytoskeleton [66–70].”
Myotubes differentiate optimally on substrates with tissue-like stiffness (2004, background): “Fibroblasts (FC7) are much softer than C2C12 cells with an AFM-determined elasticity of EFibroblast 2–5 kPa (Wu et al., 1998; Bushell et al., 1999; Rotsch and Radmacher, 2000; Mahaffy et al., 2004).”
The biomechanics toolbox: experimental approaches for living cells and biomolecules (2003, methodology): “Single cell Cytodetacher CD Measure cell-substrate adhesion forces [8] Micropipette aspiration MA Viscoelastic properties of erythrocyte cortex [9,10] Optical stretcher OS Noncontact, large deformation of cells [11] Single cell & single molecule Atomic force microscopy AFM Cell / cytoskeletal protei”
Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. (2005, background): “4 C), but does not significantly contribute to a cell9s interphase elasticity (Janmey et al., 1991; Rotsch and Radmacher, 2000).”