Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories

By C. J. Jones, Keith J. Edwards, S. Castaglione, M. Winfield, F. Sala, C. V. D. Wiel, G. Bredemeijer, B. Vosman, M. Matthes, A. Daly and 12 more. Molecular breeding, 2004

864 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/bb09d38adc098d5000439b1ad8a4f32ee04c565a

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In Genetics

1. P. Taberlet
2. K. Semagn
3. E. Bellemain
4. Å. Bjornstad
5. A. Singh
6. B. Singh
7. C. Brochmann
8. F. Pompanon

In Molecular Biology

1. D. Mackill
2. B. Collard
3. P. Taberlet
4. F. Pompanon
5. J. van Staden
6. Jinguo Hu
7. B. Vick
8. C. Brochmann

In Plant Science

1. D. Mackill
2. B. Collard
3. K. Semagn
4. A. Singh
5. B. Singh
6. Å. Bjornstad
7. C. Oliveira
8. L. Goulao

Citations in the corpus

• An overview of molecular marker methods for plants (2006, background): “It is highly reliable and reproducible (Mueller et al., 1996; Lin et al., 1996; Powell et al., 1996; Jones et al., 1997).”
• AFLP analysis of genetic diversity within and between populations of perennial ryegrass (Lolium perenne L.) (2001, methodology): “The AFLP technique (Vos et al., 1995) is particularly suitable for genetic diversity analysis due to the detection of multiple loci in a single as- say, and high reproducibility compared to the RAPD technique (Jones et al., 1997).”
• Development and characterisation of 140 new microsatellites in apple (Malus x domestica Borkh.) (2002, background): “RAPDs usually yield less than five dominant markers per PCR reaction, are difficult to reproduce (Jones et al. 1997), and are generally not transferable to progenies derived from different parents.”
• A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm (2000, methodology): “The technique is more reliable than the RAPD technique (Vos et al., 1995; Jones et al., 1997), but also more laborious and time consuming (Milbourne et al., 1997; Powell et al., 1996).”
• Variation of DNA fingerprints among accessions within maize inbred lines and implications for identification of essentially derived varieties. (2002, background): “Potential reproducibility problems were investigated by Jones et al. (1997), who reported scoring differences of up to 2 bp among SSR fragments.”
• Marker-Assisted Plant Breeding: Principles and Practices (2015, background): “Some minisatellite probes do produce low-resolution fingerprints in plants; they can be used for variety identification (Jones et al. 1997).”
• Bulked AFLP analysis for the assessment of genetic diversity in white clover (Trifolium repens L.) (2001, background): “AFLP analysis is not vulnerable to the reproducibility problems often observed with randomly amplified polymorphic DNA (RAPD) markers (Jones et al., 1997).”
• Phylogenetic relationships in Betula (Betulaceae) based on AFLP markers (2008, methodology): “We applied AFLP as an alternative, because AFLP generates polymorphic markers at a high frequency, has a high reproducibility, and has genome wide sampling and its markers are not under direct selection pressure (Jones et al. 1997; McGregor et al. 2000; Russell et al. 1997).”
• Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants (2009, background): “Reproducibility RAPD markers have been notorious for having problems with reproducibility, especially between laboratories (Hallden et al. 1996; Jones et al. 1997; Penner 1996).”
• A comparison of genetic relationship measures in strawberry (Fragaria × ananassa Duch.) based on AFLPs, RAPDs, and pedigree data (2001, background): “In addition, RAPD markers are generally considered less reproducible, or more prone to error, than AFLP markers (Jones et al., 1997).”
• Cultivar identification and genetic map of mango (Mangifera indica) (2004, methodology): “RAPD markers, which were used to identify mango cultivars (Schnell et al., 1995), are limited in their level of polymorphism and the technique is not highly reproducible (Jones et al., 1997).”