The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

By Shana Elbaum-Garfinkle, Younghoon Kim, Krzysztof Szczepaniak, Carlos Chih-Hsiung Chen, C. Eckmann, Sua Myong, C. Brangwynne. Proceedings of the National Academy of Sciences of the United States of America, 2015

1,131 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b962b4c10a5c10ac40ae2c5cba6026cfb78fdfd7

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Citations in the corpus

Phase separation by low complexity domains promotes stress granule assembly and drives pathological fibrillization (2015, positive): “Our finding that the LCD of hnRNPA1 mediates LLPS is consistent with recent reports that LCD-mediated LLPS of the DEAD-box helicases Ddx4 and Laf-1 play roles in the assembly of P granules (Elbaum-Garfinkle et al., 2015; Nott et al., 2015).”
Principles and Properties of Stress granules (2016, result): “This model has gathered support from the observation that many IDRs from RNP granule components undergo LLPS in vitro, including the RNP granule components hnRNP A1,[39,40] Ddx4, [34] LAF- 1,[42] FUS [51], and Whi3 [52].”
Spatiotemporal control of intracellular phase transitions using light-activated optoDroplets (2016, positive): “The properties of such in vitro droplets have recently been found to be malleable and time-dependent (Elbaum-Garfinkle et al., 2015; Zhang et al., 2015; Weber and Brangwynne, 2012; Molliex et al., 2015; Lin et al., 2015; Xiang et al., 2015; Patel et al., 2015), underscoring the role of IDR/LCSs in b”
G3BP–Caprin1–USP10 complexes mediate stress granule condensation and associate with 40S subunits (2016, background): “…and temperature dependence, and fusion and fission) have led to an evolving “liquid–liquid phase separation” (LLPS) model (Brangwynne et al., 2009; Elbaum-Garfinkle et al., 2015) that posits that RNA granules are composed of immis-cible liquid droplets, held apart from the cytosol by fleeting…”
Competing protein-RNA interaction networks control multiphase intracellular organization (2020, neutral): “Notwithstanding tremendous attention focused on self-associating IDRs in LLPS (Elbaum-Garfinkle et al., 2015; Kato et al., 2012; Lin et al., 2015; Molliex et al., 2015; Nott et al., 2015; Patel et al., 2015; Wang et al., 2018), studies have shown that both ODs and RBDs have essential roles in conden”
Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. (2018, neutral): “Intracellular phase transitions arise from weak, multivalent interactions, often mediated by intrinsically disordered proteins/regions (IDPs/IDRs), which are closely related to low complexity sequences and prion-like domains (Brangwynne et al., 2015; Elbaum-Garfinkle et al., 2015; Kato et al., 2012;”
RNA controls PolyQ protein phase transitions (2015, methodology): “Before adding RNA, the bulk concentration outside the protein droplet is the saturation concentration in these conditions, corresponding to point ‘‘a’’ in Figure 2C (Elbaum-Garfinkle et al., 2015; Jones, 2002).”
A molecular grammar governing the driving forces for phase separation of prion-like RNA binding proteins (2018, positive): “However, there are numerous examples of non-prion-like intrinsically disordered regions that drive phase separation such as DDX4, LAF-1 and FIB1 (Elbaum-Garfinkle et al., 2015; Feric et al., 2016; Nott et al., 2015).”
Liquid phase condensation in cell physiology and disease (2017, background): “The saturating concentration of the P granule protein LAF-1 was found to be insensitive to the presence of short [50 nucleotide (nt)] polyadenylate [poly(A)] RNA, even while the physical properties of the droplets such as the internal viscosity are strongly affected (41).”
Coactivator condensation at super-enhancers links phase separation and gene control (2018, background): “Previous studies have implicated low-complexity IDRs of proteins in liquid-liquid phase separation (7, 36, 37, 42).”
Coexisting liquid phases underlie nucleolar sub-compartments (2016, neutral): “However, given that RNA can impact the fluidity of related phase-separated droplets (Elbaum-Garfinkle et al., 2015; Zhang et al., 2015), the rate of FIB1-rich DFC aging could be impacted by the flux of newly synthesized rRNA transiting through the DFC.”