SUnSET, a nonradioactive method to monitor protein synthesis

By Enrico K. Schmidt, G. Clavarino, M. Ceppi, P. Pierre. Nature Methods, 2009

1,595 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

The Central Dogma Decentralized: New Perspectives on RNA Function and Local Translation in Neurons (2013, methodology): “Pioneering studies used metabolic labeling to demonstrate the synthesis of specific proteins such as tubulin in axons (Giuditta et al., 1968; Koenig, 2009), but the possibility that the signal arose from cell-body contamination could not be eliminated due to these technical limitations.”
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (2012, neutral): “protein synthesis, it is worth noting that this can be monitored through a nonradioactive method.(440)”
Guidelines for the use and interpretation of assays for monitoring autophagy (2012, background): “With regard to protein synthesis, it is worth noting that this can be monitored through a nonradioactive method.(324) Finally, p62 may be transcriptionally upregulated under certain conditions,(227,325) further complicating the interpretation of results.”
ALS/FTD Mutation-Induced Phase Transition of FUS Liquid Droplets and Reversible Hydrogels into Irreversible Hydrogels Impairs RNP Granule Function (2015, methodology): “678 Neuron 88, 678–690, November 18, 2015 ª2015 The Authors”