Chemically defined conditions for human iPS cell derivation and culture
By Guokai Chen, Daniel R. Gulbranson, Zhonggang Hou, Jennifer M. Bolin, Victor Ruotti, Mitchell D. Probasco, K. Smuga-Otto, S. Howden, Nicole R. Diol, Nicholas E. Propson and 5 more. Nature Methods, 2011
1,414 citations recorded by the source corpus.
We re-examine the individual components for human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces and splitting are chemically defined. A major improvement is the lack of a serum albumin component, as variations in either animal- or human-sourced albumin batches have previously plagued human ESC and iPSC culture with inconsistencies. Using this new medium (E8) and vitronectin-coated surfaces, we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives, and should be applicable to other reprogramming methods.
View Paper: https://www.semanticscholar.org/paper/79faa749c32c60343cbad39934886fabfa13ec44
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- Yan Zhang
- S. Howden
- Li-Fang Chu
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Citations in the corpus
- Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis (2013, methodology): “hESCs and iPS cells were cultured in E8 medium (42) on Matrigel-”
- Production of De Novo Cardiomyocytes: Human Pluripotent Stem Cell Differentiation and Direct Reprogramming (2012, unknown): “Most recently, Chen and colleagues achieved a breakthrough in hESC and hiPSC culture by formulating a fourth-generation, chemically defined medium (referred to as E8) that contains the growth factors FGF2 and TGF-b1 while lacking HSA and the antioxidant 2-mercaptoethanol (Chen et al., 2011).”
- Epigenomic Analysis of Multi-lineage Differentiation of Human Embryonic Stem Cells (2013, unknown): “, 2002), neural progenitor cells (Chambers et al., 2009; Chen et al., 2011), and mesenchymal stem cells (Vodyanik et al.”