Derivation of embryonic stem-cell lines from human blastocysts.

By Chad A. Cowan, I. Klimanskaya, J. McMahon, Jocelyn Atienza, J. Witmyer, J. Zucker, Shunping Wang, C. Morton, Andrew P. McMahon, D. Powers and 1 more. New England Journal of Medicine, 2004

986 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/b83fc70119b548fbd5ad03093eb3add669c8716a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

In Molecular Biology

In Physiology

Citations in the corpus

Immunogenicity of induced pluripotent stem cells (2011, positive): “After experimenting with the existing hESC lines, we found that the HUES lines, which can be passaged as single cells, are suitable for this purpose (Cowan et al., 2004).”
Long-Distance Growth and Connectivity of Neural Stem Cells After Severe Spinal Cord Injury (2012, positive): “To determine whether the ability to extend axons in vivo is a property expressed by stem cells derived from different human sources, we also tested the Harvard University Embryonic Stem cell 7 (HUES7) line (Cowan et al., 2004).”
Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “gov/)], and although 17 karyologically normal (euploid) hES cell lines derived from human blastocysts were recently generated that could be subcultured by enzymatic dissociation (87), these cells were also established on MEFs.”
Stochastic state transitions give rise to phenotypic equilibrium in populations of cancer cells. (2011, positive): “Such a high proliferation rate is implausible because even the most rapidly dividing human cells— embryonic stem cells—require at least 24 hr to complete a proliferation cycle (Cowan et al., 2004).”
Isolation of amniotic stem cell lines with potential for therapy (2007, background): “Whereas normal somatic stem cells are nontumorigenic, ES cells grow as teratocarcinomas when implanted in vivo”
Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines (2011, neutral): “…variation that exists among human pluripotent stem cell lines, three genomic assays were applied to 20 established ES cell lines (Chen et al., 2009; Cowan et al., 2004; Thomson et al., 1998) and 12 iPS cell lines that were recently derived and functionally characterized (Boulting et al., 2011).”
Control of developmental regulators by Polycomb in human embryonic stem cells. (2006, methodology): “Human embryonic stem (ES) cells were obtained from WiCell (Madison, WI; NIH Code WA09) and grown as described (Cowan et al., 2004).”
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery (2007, methodology): “Finally, we treated two human ES cell lines (HUES-3 and HUES-1”
Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 (2008, background): “DNA methylation and global gene expression profiles of two factor–induced iPS cells To further compare the two factor–induced iPS cells with human ES cell”