Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.

By Steven P. Gygi, G. Corthals, Yanni Zhang, Yvan Rochon, R. Aebersold. Proceedings of the National Academy of Sciences of the United States of America, 2000

1,340 citations recorded by the source corpus.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/b80c9d18840a4faa6affaa2b385ab43f271a9b92

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Citations in the corpus

Transport phenomena in nanofluidics (2008, background): “Turmel et al., 1990; Gygi et al., 2000; Lambert et al., 2005", and well-known DNA separation physics !”
Mass spectrometry-based proteomics (2003, background): “Systematic studies of the budding yeast Saccharomyces cerevisiae indeed revealed that typically only the most abundant proteins can be observed by this metho”
Fundamentals of Contemporary Mass Spectrometry (2007, neutral): “spectrometry in 1949 by Sommer and colleagues [56].”
Mapping Intact Protein Isoforms in Discovery Mode Using Top Down Proteomics (2011, neutral): “Here we use a liquid phase alternative to two-dimensional gels that bypasses both their low recovery and extensive workup steps before mass spectrometr”
Proteomics: the first decade and beyond (2003, background): “The increased use of MALDI-MS and ESI-MS/MS in the identification of 2DE-separated proteins showed that the incidence of comigrating proteins even in this, the highest resolving protein method known, was more prevalent than had been originally though”
Molecular Biologist's Guide to Proteomics (2002, methodology): “Examples of this method are LC (39, 55, 95, 106), reversephase LC (RP-LC) (64) and reverse-phase microcapillary LC (RP- LC) (41).”
Combining Genomics, Metabolome Analysis, and Biochemical Modelling to Understand Metabolic Networks (2001, background): “Furthermore, low abundance proteins are regularly overlooked [ 38 ] as are hydrophobic proteins, which are difficult to resolve using current 2D systems.”
Aligning gene expression time series with time warping algorithms (2001, methodology): “Progress in the development of high throughput protein level assays (Gygi et al., 1999, 2000) suggests that similar techniques will soon be used in the area of protein expression analysis.”
Proteomics by mass spectrometry: approaches, advances, and applications. (2009, background): “High-complexity large-scale proteomic samples contain thousands of proteins that can range upward of five orders of magnitude in their abundance (96).”
AN ACCURATE MASS TAG STRATEGY FOR QUANTITATIVE AND HIGH THROUGHPUT PROTEOME MEASUREMENTS (2002, background): “Furthermore, a single gene can give rise to multiple spots([5]) due to either co- or post-translationally modified forms of a protein, protein degradation intermediates, or alternative expression products (e.”