Exosome isolation: a microfluidic road-map.
By A. Liga, A. Vliegenthart, W. Oosthuyzen, J. Dear, M. Kersaudy-Kerhoas. Lab on a Chip, 2015
313 citations recorded by the source corpus.
Exosomes, first isolated 30 years ago, are nanoscale vesicles shed by most types of cells. The nucleic acid rich content of these nanoparticles, floating in virtually all bodily fluids, has great potential for non-invasive molecular diagnostics and may represent a novel therapeutic delivery system. However, current isolation techniques such as ultracentrifugation are not convenient and do not result in high purity isolation. This represents an interesting challenge for microfluidic technologies, from a cost-effective perspective as well as for enhanced purity capabilities, and point-of-care acquisition and diagnosis. In this frontier review, we present the current challenges, comment the first microfluidic advances in this new field and propose a roadmap for future developments. This review enables biologists and clinicians familiar with exosome enrichment to assess the performance of novel microfluidic devices and, equally, enables microfluidic engineers to educate themselves about this new class of promising biomarker-rich particles and the challenges arising from their clinical use.
Subfields: Cancer Research, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/b809067df34e5ec7195a6b127507946cc157baaa
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Citations in the corpus
- Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics (2020, background): “Major roadblocks to clinical applications include standardization, scalability, and validation [127, 159].”
- Isolation of exosomes from whole blood by integrating acoustics and microfluidics (2017, methodology): “Other methods, including immunoaffinity capture (19, 20), precipitation kits such as ExoQuick (System Biosciences) and Total Exosome Isolation (Invitrogen) (12, 21), microfluidics (17, 22, 23), nanoscale lateral displacement arrays (24), nanostructurebased filtration (25), nanoplasmonic chip (26), m”
- Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis (2019, background): “One challenge with ultrafiltration is the clogging and trapping of vesicles (and therefore loss of exosomes) on the filter unit [116].”
- Using exosomes, naturally-equipped nanocarriers, for drug delivery (2015, methodology): “There are three main techniques for isolating exosomes or microvesicles using microfluidics: (A) immunoaffinity, (B) sieving, and (C) trapping exosomes on porous structures [32].”