Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)

By Itthayakorn Promputtha, R. Jeewon, Saisamorn Lumyong, E. McKenzie, K. D. Hyde. 2005

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Citations in the corpus

DGGE coupled with ribosomal DNA gene phylogenies reveal uncharacterized fungal phylotypes (2006, methodology): “, 2005), Magnolia liliifera (Promputtha et al., 2005a), grasses (Bacon and White, 1994), palms (Fröhlich et al.”
Endophytic Pestalotiopsis species associated with plants of Podocarpaceae, Theaceae and Taxaceae in southern China (2007, methodology): “Further isolation was carried out using single spore following the methods by Choi (1999) and Promputtha et al. (2005).”
Transient leaf endophytes are the most active fungi in 1-year-old beech leaf litter (2017, background): “…with host plants (Smith et al. 2017), growing evidence suggests that some endophytic fungi become saprobes after leaf fall (Guo et al. 2001, 2003; Promputtha et al. 2002, 2005, 2007, 2010; Shirouzu et al. 2009; Suryanarayanan 2013; Saikkonen et al. 2015; Szink et al. 2016; Promputtha et al.…”
AJOM new records and collections of fungi: 1–100 (2020, background): “Many others live in symbiotic associations with plants and exhibit endophytic lifestyles (Promputtha et al. 2005, 2007, Doilom et al. 2017a, Tibpromma et al. 2018a).”
Fungal endophytes in xylem of healthy chilean trees and their possible role in early wood decay (2008, methodology): “In future studies we should use molecular techniques to aid identification of isolates or even detect not cultural isolates in healthly xylem (see Promputtha et al., 2005, 2007; Wei et al., 2005; Duong et al., 2006).”
The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens (2011, background): “Promputtha et al. (2005) reported that, from a total of 31 morphospecies of sterile endophytes from Magnolia liliflora (Magnoliaceae) identified based on molecular phylogeny, 24 were Phomopsis species; this finding has been corroborated in several other recent studies with different hosts (Murali…”
Diversity of fungal endophytes in shrubby medicinal plants of Malnad region, Western Ghats, Southern India (2008, methodology): “Molecular techniques such as ITS sequence analyses (Promputtha et al. 2005; Wang et al. 2005) and DGGE (denaturing gradient gel electrophoresis) have now been used effectively for the phylogenetic classification of morphospecies of endophytes (Jeewon & Hyde 2006), and Hambleton & Sigler (2005)…”
Diversity of endophytic fungi of Taxus globosa (Mexican yew) (2011, methodology): “Phylogenetic analysis of rDNA sequences have been successfully employed for the identification of different morphospecies (Guo et al. 2001, 2003; Promputtha et al. 2005; Wang et al. 2005, 2008; Liu et al. 2009).”
Biology of Endophytic Fungi (2012, background): “…concerning identification of endophytic fungi are that some of endophytes could not be identified to species or genus level (Gamboa & Bayman 2001, Promputtha et al. 2005a & b), and having many non-identified mycelia sterilia, raises the importance of use modern molecular techniques that could be…”
Molecular characterization of fungal endophytic morphospecies isolated from stems and pods of Theobroma cacao (2006, background): “Fifty-nine of these morphologically unidentifiable isolates were selected for molecular identification by DNA extraction and sequence analysis of nuclear ribosomal DNA (rDNA).”
Can leaf degrading enzymes provide evidence that endophytic fungi becoming saprobes? (2010, methodology): “Endophytes and saprobes from Magnolia liliifera Fungal cultures used in this study were isolated as endophytes and saprobes from studies on M. liliifera and methods have been discussed in detail (Promputtha et al. 2002, 2004, 2005, 2007).”
Endophytic fungal diversity: review of traditional and molecular techniques (2012, unknown): “(2006) recovered 14 OTUs from Magnolia liliifera using DGGE coupled with sequencing, which were totally different from the morphospecies recovered by the traditional cultivationdependent method (Promputtha et al. 2005).”
Phylogenetic diversity of endophytic Pestalotiopsis species in Pinus armandii and Ribes spp.: evidence from rDNA and β- tubulin gene phylogenies (2007, methodology): “Isolation of endophytes from tissues was carried out by modifying the methods outlined by Kumar and Hyde (2004), Wei and Xu (2004) and Promputtha et al. (2005).”
Diversity of saprobic microfungi (2007, background): “many endophytic and pathogenic fungi may persist as saprobes once the plant organ, on which they reside, senesces (Zhou and Hyde 2001; Photita et al. 2004, 2005; Promputtha et al. 2005).”
Fungi from peatlands (2007, methodology): “Marasmius salalis Desjardin & Redhead Bog Canada S Roberts et al. (2004) Melanotus horizontalis (Bull.”
Community composition of endophytic fungi in Acer truncatum and their role in decomposition (2010, background): “Colletotrichum [anamophic Glomerella] and Phomopsis [anamophic Diaporthe]) were isolated from leaves of Magnolia liliifera in Thailand (Promputtha et al. 2005), and these fungi were also recovered Table 5 Extracellular enzyme activities of endophytic fungi from Acer truncatum”