Highly purified Th17 cells from BDC2.5NOD mice convert into Th1-like cells in NOD/SCID recipient mice.

By D. Bending, Hugo De la Peña, M. Veldhoen, J. Phillips, C. Uyttenhove, Brigitta Stockinger, A. Cooke. Journal of Clinical Investigation, 2009

599 citations recorded by the source corpus.

Th17 cells are involved in the pathogenesis of many autoimmune diseases, but it is not clear whether they play a pathogenic role in type 1 diabetes. Here we investigated whether mouse Th17 cells with specificity for an islet antigen can induce diabetes upon transfer into NOD/SCID recipient mice. Induction of diabetes in NOD/SCID mice via adoptive transfer of Th1 cells from BDC2.5 transgenic mice was prevented by treatment of the recipient mice with a neutralizing IFN-γ-specific antibody. This result suggested a major role of Th1 cells in the induction of disease in this model of type 1 diabetes. Nevertheless, transfer of highly purified Th17 cells from BDC2.5 transgenic mice caused diabetes in NOD/SCID recipients with similar rates of onset as in transfer of Th1 cells. However, treatment with neutralizing IL-17-specific antibodies did not prevent disease. Instead, the transferred Th17 cells, completely devoid of IFN-γ at the time of transfer, rapidly converted to secrete IFN-γ in the NOD/SCID recipients. Purified Th17 cells also upregulated Tbet and secreted IFN-γ upon exposure to IL-12 in vitro and in vivo in NOD/SCID recipients. These results indicate substantial plasticity of Th17 commitment toward a Th1-like profile.

Subfields: Genetics, Immunology

View Paper: https://www.semanticscholar.org/paper/196083d13bdd7c7ec34c3c774c60740aac80a08d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. D. Mathis
2. Dan R. Littman
3. I. Ivanov
4. C. Benoist

In Immunology

1. Dan R. Littman
2. Hsin-jung Wu
3. D. Mathis
4. C. Benoist
5. Alexander Y. Rudensky
6. T. Malek
7. I. Castro
8. K. Honda

Citations in the corpus

• Gut-residing segmented filamentous bacteria drive autoimmune arthritis via T helper 17 cells (2010, methodology): “Asterisks indicate statistical significance via the Student's t test, *p < 0.05. diseases has there emerged definitive evidence of a critical role for Th17 cells (Devoss et al., 2008; Martin-Orozco et al., 2009; Bending et al., 2009; Emamaullee et al., 2009).”
• CD4+T Cells: Differentiation and Functions (2012, background): “Th17 in the presence of IL12 switched to Th1 phenotype, and interaction with IL4 led to the differentiation into Th2 cells [109, 110].”