Universal sample preparation method for proteome analysis
By C. Eyers. 2009
3,034 citations recorded by the source corpus.
View Paper: https://www.semanticscholar.org/paper/b34e3cee8197c96640251c92b7ab5262a05874d6
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- Jüergen Cox
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- Jacek R. Wisniewski
Citations in the corpus
- Deep proteome and transcriptome mapping of a human cancer cell line (2011, methodology): “In all, 0.100ml of the cell lysate containing 10mg of total protein was loaded onto a Superdex 200 10/300 GL column (GE Healthcare BioSciences AB, Uppsala) equilibratedwith TNS buffer composed of 0.1M Tris–HCl, pH 8 buffer, 0.1M NaCl and 0.2% SDS. Proteins were eluted with TNS buffer and 2ml fractio”
- Decoding signalling networks by mass spectrometry-based proteomics (2010, background): “Current depth of analysis is about 7,000 proteins with a 48 hour measurement tim”
- Exosomes as Critical Agents of Cardiac Regeneration Triggered by Cell Therapy (2014, methodology): “Proteins were prepared for digestion using the filter-assisted sample preparation (FASP) method (Wi sniewski et al., 2009).”
- Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. (2014, methodology): “For proteome analysis, 30 mg of the peptides was separated on a pipette-tip based SAX column (Wi sniewski et al., 2009a).”
- Ultrasensitive proteome analysis using paramagnetic bead technology (2014, unknown): “Although SDS-Gels (Schmidt, 2013), FASP (Yu et al , 2014), in-solution, and iST (Kulak et al , 2014) methods are theoretically scalable to 96-well format, these protocols can require specialized equipment (iST) and lengthy processing (FASP) or perform poorly with small quantities of sample (FASP, SD”
- Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration (2017, methodology): “1 MDTT (Merck)) and all liquid added on top of a spin-filter (10 kDa, PALL, Port Washington, NY) for a filter-aided sample preparation following a previous protocol (Wiśniewski et al., 2009) where 6M GuHCl was used instead of urea.”