Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.

By Michael Walter, Christina Chaban, Katia Schütze, Oliver Batistič, Katrin Weckermann, Christian Näke, Dragica Blazevic, Christopher Grefen, K. Schumacher, C. Oecking and 2 more. The Plant Journal, 2004

1,627 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/01fd9053bd9a998bc626a40603912ca4dbc0f824

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Citations in the corpus

Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. (2009, methodology): “Construction of the pUCSPYNE- and pUCSPYCE-based plasmids for BiFC analysis ( Walter et al. 2004 ) is described in the Supplementary data.”
A Novel Role for the TIR Domain in Association with Pathogen-Derived Elicitors (2007, methodology): “As a control to show that the two portions of Citrine could reconstitute fluorescence, published interactions using the 14-3-3 protein, T14-3c, which is known to homodimerize, were repeated [41] (Figure S1A).”
The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples. (2012, methodology): “…MdbHLH33 and MdMYB31 were cloned into pSPYNE-35S and pSPYCE-35S, which contain DNA encoding the N or C-terminal regions of YFP (YFPN or YFPC), respectively, to generate the plasmids MdbHLH3/MdMYB1/MdbHLH33YFPN and MdbHLH3/MdMYB1/MdMYB31-YFPC according to previous protocols (Walter et al. 2004).”
Gateway-compatible vectors for plant functional genomics and proteomics. (2006, background): “Walter et al. also describe Gateway-compatible vectors that facilitate BiFC assays, in which non-fluorescent N- and C-terminal fragments of YFP must dimerize to reconstitute YFP fluorescence (Walter et al., 2004).”
A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes (2011, methodology): “Plasmids pUCSPYNE and pUC-SPYCE were described previously [30].”
Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants1[C][W][OA] (2007, methodology): “BiFC is relatively simple compared to FRET and BRET and has been used in a number of plant protein-protein interaction studies (Bracha-Drori et al., 2004; Walter et al., 2004; Dong et al., 2006; Quan et al., 2007).”
Gateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene Construction (2012, methodology): “Some vectors are developed to add the epitope tag to split fragments so that the detection of accumulation of fusion proteins is carried out by immunoblotting (Bracha-Drori et al., 2004; Waadt et al., 2008; Walter et al., 2004).”
Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. (2008, positive): “Among higher eukaryotic organisms, BiFC analysis has been used to visualize numerous interactions in many plant species (1, 5, 11, 12, 14, 17, 18, 24, 25, 40, 51, 57, 58, 62, 65, 69, 70, 81, 86, 88, 92, 100, 106, 112, 116, 117, 122, 123).”
Fluorescence lifetime imaging microscopy in life sciences (2010, methodology): “An alternative fluorescence method for monitoring protein interactions is based on the bimolecular fluorescence complementation (BiFC) technology [82–84].”
A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ transporter AKT1 in Arabidopsis. (2006, methodology): “BiFC assays were performed as described previously (Walter et al., 2004).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, positive): “Split yellow fluorescence protein (YFP) for protein interaction analysis For the in vivo protein interaction, the bimolecular fluorescence complementation (BiFC) method described by Walter et al. (2004) was adapted.”
Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis (2009, methodology): “The cDNAs of SRK2E, SRK2I, and ABI1 were cloned into pUC-SPYNEGW or pUCSPYCEGW (33).”