Xenopus Cdc45‐dependent loading of DNA polymerase α onto chromatin under the control of S‐phase cdk

By S. Mimura, H. Takisawa. 1998

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Citations in the corpus

• In silico protein interaction screening uncovers DONSON’s role in replication initiation (2023, methodology): “Rabbit polyclonal antibodies against the following proteins were used as primary antibodies for Western blotting: DONSON (1:5000, described above) MCM7 [1:12,000, (31)] MCM4 (1:4000, Bethyl #A300-193A, RRID: AB_162720) MCM3 (1:4000, SantaCruz (H-215) #sc-292857) CDC45 [1:20,000, (56)] GINS [1:5000, ”
• The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication (2002, background): “At the G1/S transition, the pre-RC is converted to a preinitiation complex (pre-IC) through the actions of two protein kinases, S phase–specific cyclin dependent kinase (S-Cdk) and the Cdc7-Dbf4 protein kinase, which cooperate to recruit the initiation factor Cdc45 (Mimura and Takisawa, 1998; Zou an”
• The N-Terminal Noncatalytic Region of Xenopus RecQ4 Is Required for Chromatin Binding of DNA Polymerase α in the Initiation of DNA Replication (2006, methodology): “The egg extracts were immunoprecipitated as described previously ( 25 ).”
• Treslin Collaborates with TopBP1 in Triggering the Initiation of DNA Replication (2010, background): “Mcm2 (a marker for the MCM complex) preceded Treslin in associating with chromatin , whereas Cdc45 commenced binding just after Treslin.”
• Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha. (2000, methodology): “Thus, RPA depletion and camptothecinproposed (Mimura and Takisawa, 1998).”
• MTBP, the partner of Treslin, contains a novel DNA-binding domain that is essential for proper initiation of DNA replication (2017, background): “The association of Cdc45 with chromatin correlates closely with activation of the replicative helicase and initiation of replication in egg extracts (Mimura and Takisawa, 1998; Mimura et al., 2000; Walter and Newport, 2000).”
• The cell cycle and seed germination (2003, background): “This enzyme, in turn, is loaded to the DNA by the product of the cdc45 gene (Mimura and Takisawa, 1998).”
• Mcm10 and And-1/CTF4 recruit DNA polymerase α to chromatin for initiation of DNA replication (2007, background): “…with and is required to load Mcm10, which is itself critical for the loading of replication elongation factors like Cdc45 (Aparicio et al. 1997), GINS, and DNA polymerase (pol ) (Mimura and Takisawa 1998; Takayama et al. 2003; Ricke and Bielinsky 2004; Gambus et al. 2006; Pacek et al. 2006).”