Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila.

By D. Wagh, Tobias M. Rasse, E. Asan, A. Hofbauer, Isabel Schwenkert, Heike Dürrbeck, S. Buchner, M. Dabauvalle, Manuela Schmidt, Gang Qin and 4 more. Neuron, 2006

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Subfields: Cell Biology, Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ad264da617934f45765a28f641c653fa3fe34b46

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Citations in the corpus

Organization of antennal lobe‐associated neurons in adult Drosophila melanogaster brain (2012, background): “It recognizes a ubiquitously expressed active zone protein, Bruchpilot, which forms protein bands of 190 and 170 kDa in the western blots of homogenized Drosophila heads (Wagh et al., 2006).”
Structure of the adult central complex in Drosophila: Organization of distinct neuronal subsets (2010, background): “The nc82 antibody recognizes the protein Bruchpilot, which forms protein bands of 190 and 170 kDa in the Western blots of homogenized Drosophila heads (Wagh et al., 2006).”
Non-Canonical Odor Coding in the Mosquito (2020, positive): “Purification of nc82/Brp monoclonal antibody Hybridoma cells expressing monoclonal antibody nc82 (Antibody Registry ID: AB_2314866), which recognizes the Drosophila melanogaster Brp protein (Wagh et al., 2006) developed by Erich Buchner were obtained from the Developmen-”
A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster (2017, neutral): “(B) The LM template brain is labeled with nc82 (magenta), a synapse-specific antibody commonly used to reveal neuropil compartment boundaries (Wagh et al., 2006).”
Induction of aversive learning through thermogenetic activation of Kenyon cell ensembles in Drosophila (2014, methodology): “The following antibodies were used: mouse anti-nc82 against Bruchpilot (Wagh et al., 2006) (provided by Erich Buchner) diluted 1:10, rat anti-RFP (5F8, Chromotec) to stain mCherry diluted 1:300, and rabbit antiGFP (A6455, Invitrogen) diluted 1:200.”
Neuronal assemblies of the Drosophila mushroom body (2008, methodology): “The immunoreactive signal disappears in the tissue as well as in the Western blots if the bruchpilot gene is knocked down, and an additional band is detected specifically if GFP-tagged Bruchpilot is expressed in a panneuronal manner (Wagh et al., 2006).”
Development‐based compartmentalization of the Drosophila central brain (2010, background): “Compartments can be easily visualized by global markers for neurites or synapses; the most widely used of these markers are antibodies against the synaptic protein Brp (nc82; Wagh et al., 2006) and Ncadherin (DNcad; Iwai et al., 1997).”
Negative Regulation of Active Zone Assembly by a Newly Identified SR Protein Kinase (2009, methodology): “Recently, it was demonstrated that mutations in the brp gene eliminate T-bars and severely impair synaptic vesicle release, consistent with the conclusion that T-bars and Brp are essential components of the presynaptic AZ [4,5].”
LAR, liprin α and the regulation of active zone morphogenesis (2007, background): “bruchpilot mutants have severe electrophysiological, behavioral and morphological defects (Kittel et al., 2006; Wagh et al., 2006); they lack the conspicuous electron-dense T-bar structures found in Drosophila active zones, have improper calcium channel localization, and diminished evoked responses ”
Bruchpilot, A Synaptic Active Zone Protein for Anesthesia-Resistant Memory (2011, background): “, 2006), we suppressed Brp expression in a targeted group of cells with a transgenic RNA interference approach (Wagh et al., 2006).”
Synaptic organization in the adult Drosophila mushroom body calyx (2009, background): “The antibody recognizes the active zone protein Bruchpilot, which forms protein bands of 190 and 170 kDa in Western blots of homogenized Drosophila heads (Wagh et al., 2006).”
Cell-type Specific Labeling of Synapses in vivo through Synaptic Tagging with Recombination (STaR) (2014, methodology): “Within the Drosophila visual system, the number and distribution of synapses correlate with electron microscopy studies.”
A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila (2006, background): “The pre-synaptic marker mouse anti-nc82 Ab can be used to label glomerular structure in the antennal lob”
A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body (2011, neutral): “We also labeled presynaptic active zones in APL and DPM neurons by expressing a uas-Bruchpilot::GFP [32] with a mCD8::mCherry [33] transgene that should label the entire cell surface.”
A GAL4-driver line resource for Drosophila neurobiology. (2012, positive): “Theywerethenincubatedinblockingbuffer(3%normal goat serum [Invitrogen] in PAT) for 1 hr at room temperature after which the blocking buffer was replaced with 1 ml of a mixture of two primary antibodies in blocking buffer: rabbit anti-GFP (Invitrogen A11122) at 1:1,000 and mouse nc82 supernatant (Dev”