Glucosinolate Metabolites Required for an Arabidopsis Innate Immune Response

By Nicole K. Clay, A. M. Adio, Carine Denoux, Georg Jander, Frederick M. Ausubel. Science, 2008

1,063 citations recorded by the source corpus.

The perception of pathogen or microbe-associated molecular pattern molecules by plants triggers a basal defense response analogous to animal innate immunity and is defined partly by the deposition of the glucan polymer callose at the cell wall at the site of pathogen contact. Transcriptional and metabolic profiling in Arabidopsis mutants, coupled with the monitoring of pathogen-triggered callose deposition, have identified major roles in pathogen response for the plant hormone ethylene and the secondary metabolite 4-methoxy-indol-3-ylmethylglucosinolate. Two genes, PEN2 and PEN3, are also necessary for resistance to pathogens and are required for both callose deposition and glucosinolate activation, suggesting that the pathogen-triggered callose response is required for resistance to microbial pathogens. Our study shows that well-studied plant metabolites, previously identified as important in avoiding damage by herbivores, are also required as a component of the plant defense response against microbial pathogens.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/ac9b1d347be0c304c1b306a6042e63cf156f1944

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. B. Mauch-Mani
2. J. Ton
3. V. Flors
4. Islam Mostafa
5. Frederick M. Ausubel
6. V. Pastor
7. Zhenyu Cheng
8. P. Dodds

In Plant Science

1. B. Mauch-Mani
2. V. Pastor
3. V. Flors
4. Estrella Luna
5. J. Ton
6. J. Rathjen
7. P. Dodds
8. Yuelin Zhang

Citations in the corpus

• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “In pl nt cells, perception of conserved pathogen-associated molecular patterns (PAMPs) leads to activatio of a battery of defence mech nisms, including the prod ction of reactive oxygen species, the synthesis of antibiotic phytoalexins, d posi ion of callose, and expression of defence-re ated gen s ”
• Glucosinolate Biosynthesis and the Glucosinolate–Myrosinase System in Plant Defense (2020, background): “, anti-carcinogenesis) [7–9], potential functions in non-host resistance to bacteria and fungi [10,11] and insect defense [3,12–14].”
• The Apoplastic Oxidative Burst Peroxidase in Arabidopsis Is a Major Component of Pattern-Triggered Immunity[W][OA] (2012, methodology): “…of several genes previously shown to be required for callose deposition, including the transcription factor geneMYB51 and two cytochrome P450-encoding genes required for the synthesis of methoxylated IGSs (Clay et al., 2009; Millet et al., 2010), are downregulated in the peroxidase knockdown lines.”
• The multifaceted role of ABA in disease resistance. (2009, positive): “can suppress bacteria-induced callose deposition and, therefore, contribute negatively to resistance [14, 16 ].”
• Plant Protection by Benzoxazinoids—Recent Insights into Biosynthesis and Function (2018, background): “Glucosinolates (GS) in the family Brassicaceae share features with BXs: their biosynthesis is tightly linked to tryptophan and hence auxin biosynthesis, GS are expressed constitutively at developmental stages but the pattern can be changed by biotic and abiotic stress, and both play an essential rol”
• Receptor-like cytoplasmic kinases integrate signaling from multiple plant immune receptors and are targeted by a Pseudomonas syringae effector. (2010, methodology): “A seedling-based procedure for callose induction was adapted from Clay et al. (2009).”
• Callose deposition: a multifaceted plant defense response. (2011, methodology): “The recent discovery that glucosinolate metabolites regulate Flg22-induced callose adds a novel layer to signaling pathways controlling this PTI response (Clay et al. 2009).”
• Plant immunity: towards an integrated view of plant–pathogen interactions (2010, background): “PEN2 is a hydrolytic enzyme that produces a glucosinylate compound from an inactive precursor and PEN3 is an ABC transporter that seems to be involved in secretion of this molecule at the site of fungal attac”