Callose deposition: a multifaceted plant defense response.

By Estrella Luna, V. Pastor, J. Robert, V. Flors, B. Mauch-Mani, J. Ton. Molecular Plant-Microbe Interactions, 2011

681 citations recorded by the source corpus.

Callose deposition in Arabidopsis has emerged as a popular model system to quantify activity of plant immunity. However, there has been a noticeable rise in contradicting reports about the regulation of pathogen-induced callose. To address this controversy, we have examined the robustness of callose deposition under different growth conditions and in response to two different pathogen-associated molecular patterns, the flagellin epitope Flg22 and the polysaccharide chitosan. Based on a commonly used hydroponic culture system, we found that variations in growth conditions have a major impact on the plant's overall capacity to deposit callose. This environmental variability correlated with levels of hydrogen peroxide (H₂O₂) production. Depending on the growth conditions, pretreatment with abscissic acid stimulated or repressed callose deposition. Despite a similar effect of growth conditions on Flg22- and chitosan-induced callose, both responses showed differences in timing, tissue responsiveness, and colocalization with H₂O₂. Furthermore, mutant analysis revealed that Flg22- and chitosan-induced callose differ in the requirement for the NADPH oxidase RBOHD, the glucosinolate regulatory enzymes VTC1 and PEN2, and the callose synthase PMR4. Our study demonstrates that callose is a multifaceted defense response that is controlled by distinct signaling pathways, depending on the environmental conditions and the challenging pathogen-associated molecular pattern.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/8804fd204d3c4c5b36eb1c3bc6319d0a984b99ee

How researchers see this paper

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In Plant Science

1. A. Venkateswarlu
2. V. Pastor
3. B. Mauch-Mani
4. S. Fraire-Velázquez
5. I. Ben Rejeb

Citations in the corpus

• Abiotic and Biotic Stress Response Crosstalk in Plants (2011, background): “This demonstrates that callose deposition is a multifaceted response controlled by multiple signaling pathways, depending of the environmental conditions and the challenging pathogen-associated molecular pattern (Luna et al., 2011).”
• Plant Responses to Simultaneous Biotic and Abiotic Stress: Molecular Mechanisms (2014, background): “However, recent findings show a positive effect of ABA on biotic stress resistance [30,104,105].”
• Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives (2011, background): “This demonstrates that callose deposition is a multifaceted response controlled by multiple signaling pathways, depending of the environmental conditions and the challenging pathogen-associated molecular pattern (Luna et al., 2011).”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “Many plant species react to pathogen or insect attack by synthesizing lignin and callose that serve as a protective physical barrier (Matern and Kneusel, 1988; Bhuiyan et al., 2009; Luna et al., 2010).”