The Apoplastic Oxidative Burst Peroxidase in Arabidopsis Is a Major Component of Pattern-Triggered Immunity[W][OA]

By OA W, A. Daudi, Zhenyu Cheng, J. O’Brien, Nicole D. Mammarella, Safina Khan, Frederick M. Ausubel, G. P. Bolwella. The Plant Cell, 2012

595 citations recorded by the source corpus.

This article examines the role of apoplastic peroxidases in Arabidopsis thaliana pattern-triggered immunity (PTI). The reduced expression of two key peroxidase genes, PRX33 or PRX34, causes defects in PTI, and an rbohD mutant is also impaired in some PTI-related responses, but to a lesser degree than the peroxidase knockdown lines. In plants, reactive oxygen species (ROS) associated with the response to pathogen attack are generated by NADPH oxidases or apoplastic peroxidases. Antisense expression of a heterologous French bean (Phaseolus vulgaris) peroxidase (FBP1) cDNA in Arabidopsis thaliana was previously shown to diminish the expression of two Arabidopsis peroxidases (peroxidase 33 [PRX33] and PRX34), block the oxidative burst in response to a fungal elicitor, and cause enhanced susceptibility to a broad range of fungal and bacterial pathogens. Here we show that mature leaves of T-DNA insertion lines with diminished expression of PRX33 and PRX34 exhibit reduced ROS and callose deposition in response to microbe-associated molecular patterns (MAMPs), including the synthetic peptides Flg22 and Elf26 corresponding to bacterial flagellin and elongation factor Tu, respectively. PRX33 and PRX34 knockdown lines also exhibited diminished activation of Flg22-activated genes after Flg22 treatment. These MAMP-activated genes were also downregulated in unchallenged leaves of the peroxidase knockdown lines, suggesting that a low level of apoplastic ROS production may be required to preprime basal resistance. Finally, the PRX33 knockdown line is more susceptible to Pseudomonas syringae than wild-type plants. In aggregate, these data demonstrate that the peroxidase-dependent oxidative burst plays an important role in Arabidopsis basal resistance mediated by the recognition of MAMPs.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/b5e2511a30bc61ed4f47f7d9c262739d35f8924c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. D. Gupta
2. A. Sharma
3. J. Palma
4. Dr. Ishwar Prakash Sharma
5. F. J. Corpas

In Plant Science

1. F. J. Corpas
2. D. Gupta
3. A. Sharma
4. J. Palma
5. Dr. Ishwar Prakash Sharma

Citations in the corpus

• Antioxidants and Antioxidant Enzymes in Higher Plants (2018, background): “The knockdown of AtPrx33 and AtPrx34 transcripts by transduction of antisense FBP1 from Phaseolus vulgaris into A. thaliana resulted in a failed oxidative burst and an increased sensitivity to fungal and bacterial pathogens (Daudi et al. 2012).”
• Physiological and biochemical changes in tomato cultivar PT-3 with dual inoculation of mycorrhiza and PGPR against root-knot nematode (2016, background): “Further, PO-dependent ROS are well known to be involved in deposition of callose and in upregulation of defense genes (Daudi et al. 2012; Wrzaczek et al. 2013).”