DNA isolation from dry and fresh samples of polysaccharide-rich plants

By Arun Dev Sharma, P. Gill, P. Singh. Plant Molecular Biology Reporter, 2002

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Two alleles of ahFAD2B control the high oleic acid trait in cultivated peanut. (2009, methodology): “The method of DNA extraction from seeds followed a protocol for Southern blot with minor modifi cations (Sharma et al., 2002).”
Semi-automated, Membrane-Based Protocol for DNA Isolation from Plants (2008, background): “…to break the cell wall, the incomplete removal of polysaccharides or secondary metabolites, such as polyphenols, during DNA extraction often inhibits enzymatic reactions (i.e. PCR) and causes DNA degradation after long-term storage (Porebski et al. 1997; Schlink and Reski 2002; Sharma et al. 2002).”
Molecular marker based adulteration detection in traded food and agricultural commodities of plant origin with special reference to spices. (2010, background): “(39); commercial samples of tea (40); cylinder of sugar cane (41); fresh and dry leaves of medicinal plants (42-44); poppy seeds (45); peanut (46 ); potato tubers (47); dried corn cobs (48); chick pea seeds, soybean (49); mature fresh rhizomes of ginger and turmeric (50); jams and yoghurts (51); oli”