Bisulfite genomic sequencing: systematic investigation of critical experimental parameters

By C. Grunau, Susan J. Clark, A. Rosenthal. Nucleic Acids Res., 2001

774 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis (2005, background): “It has been estimated that >90% of the input DNA is lost to DNA degradation during the first hour of a bisulfite reaction (38).”
Transposable Elements: Targets for Early Nutritional Effects on Epigenetic Gene Regulation (2003, methodology): “Hence, by digesting genomic DNA with AloI and employing reverse primers specific to PS1A, bisulfite sequencing (9) was used to quantify site-specific CpG methylation of the A PS1A in A/a mice.”
Incomplete DNA methylation underlies a transcriptional memory of the somatic cell in human iPS cells (2011, positive): “Total genomic DNA underwent bisulphite conversion following an established protoco”
CpG island hypermethylation and tumor suppressor genes: a booming present, a brighter future (2002, background): “At this point, special attention must also be paid to the influence of the temperature and incubation time on DNA integrity during the bisulfite treatment since, under these conditions, 84 – 96% of the DNA is degraded (Grunau et al., 2001).”
QUMA: quantification tool for methylation analysis (2008, methodology): “To align bisulfite sequences with a genomic sequence, we took a pairwise-alignment approach for QUMA, instead of the multiple-alignment approach used by some other programs (6,10,13,14).”