Running head: Analyses of Expressed Sequence Tags from Apple.

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Identification and stability of QTLs for fruit quality traits in apple (2008, background): “For these reasons, genetic linkage maps containing microsatellites (simple sequence repeats (SSRs)) are indispensable in MAS and/or MAB programmes and further developments in this direction will undoubtedly be aided by the recent availability of large public Malus expressed sequence tag (EST) collec”
Genome mapping of three major resistance genes to woolly apple aphid (Eriosoma lanigerum Hausm.) (2008, unknown): “In addition, a new set of microsatellite markers was developed from apple expressed sequence tags (Newcomb et al. 2006).”
Flavonoid genes of pear (Pyrus communis) (2007, background): “Considering the current number of accessions of the genera Pyrus, Malus, Prunus, Fragaria, and Rosa in GenBank (Table 4), it is obvious that Malus sequences are the best source of sequence information for Pyrus gene cloning, especially by assembling Malus expressed sequence tags (ESTs) (Newcomb et a”
The genome of the domesticated apple (Malus × domestica Borkh.) (2010, background): “In Rosaceae, photosynthesis-derived carbohydrates are transported mainly as sorbito”
Characteristics and transferability of new apple EST-derived SSRs to other Rosaceae species (2009, background): “%) of putative EST–SSRs present in apple (Newcomb et al. 2006), it is likely that some additional individual apple EST–SSRs will yield high frequencies of transferability across Rosaceae.”