Comprehensive proteomic analysis of the human spliceosome

By Zhaolan Zhou, L. Licklider, Steven P. Gygi, R. Reed. Nature, 2002

938 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/a8dd5ef544884e6472fa9dcb938417a1a327f493

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In Molecular Biology

Citations in the corpus

Human Splicing Finder: an online bioinformatics tool to predict splicing signals (2009, background): “During this event, parts of the pre-mRNA transcripts are removed in a ribonucleoprotein complex (spliceosome) which is constituted of five essential small nuclear RNAs and more than 150 polypeptides (2,3).”
Nuclear speckles: a model for nuclear organelles (2003, result): “Consistent with these findings, the most recent proteomic analyses of in-vitro -assembled spliceosomes indicate that they might also contain transcription and 3′-end RNA-processing factors, together with splicing factors, in a higher-order comple”
The spliceosome: design principles of a dynamic RNP machine. (2009, background): “Initial mass spectrometric analyses of a mixed population of affinity-purified spliceosomal complexes indicated that between 150 (Zhou et al., 2002) and 300 distinct proteins (Rappsilber et al., 2002) copurify with spliceosomes.”
Opportunities and challenges Genome-wide analyses of alternative splicing in plants: (2008, neutral): “Most intron splicing is carried out by the spliceosome, a large macromolecular machine composed of five small nuclear riboproteins (snRNPs) and numerous accessory proteins (Staley and Guthrie 1998; Zhou et al. 2002).”
Variation in alternative splicing across human tissues (2004, methodology): “For this trans-factor analysis, we obtained a list of 20 splicing factors of the SR, SR-related and hnRNP protein families from proteomic analyses of the human spliceosome [46-48] (see Materials and methods for the list of genes).”
Splicing regulation: from a parts list of regulatory elements to an integrated splicing code. (2008, background): “Containing more than 100 core proteins and five small nuclear RNAs (snRNAs U1, U2, U4, U5, and U6), spliceosomes may be the most complex machines in the cell (Zhou et al. 2002; Jurica and Moore 2003; Nilsen 2003).”
PDZ Domain Proteins: Plug and Play! (2003, background): “At the same time, it is now recognized that multiprotein nanomachines consisting of more than 100 proteins are commonplace [for instance, the splicesome ( 93 )].”
Comparative analysis identifies exonic splicing regulatory sequences--The complex definition of enhancers and silencers. (2006, background): “The splicing reaction is catalyzed by the spliceosome—a complex that contains five small nuclear ribonucleoproteins (snRNPs) and at least 150 additional proteins (Hartmuth et al., 2002; Jurica and Moore, 2003; Nilsen, 2003; Zhou et al., 2002).”