Transcriptome-wide mapping of N6-methyladenosine by m6A-seq based on immunocapturing and massively parallel sequencing
By D. Dominissini, S. Moshitch-Moshkovitz, M. Salmon-Divon, N. Amariglio, G. Rechavi. Nature Protocols, 2013
597 citations recorded by the source corpus.
Subfields: Cancer Research, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/a7c2688b3ffe52105179ffa37d18dd7a8ae415de
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In Cancer Research
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In Molecular Biology
Citations in the corpus
- Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia (2019, methodology): “m6A-seq assays were carried on according to the published protocol (Dominissini et al., 2013) with minor modifications.”
- m6A RNA Methylation Regulates the Self-Renewal and Tumorigenesis of Glioblastoma Stem Cells (2017, methodology): “m(6)A-Modified mRNAs Are Involved in Critical Cellular Processes To investigate the m(6)A modifications in the GSC transcriptome, m(6)A-seq analysis was performed as described previously (Dominissini et al., 2013).”
- METTL3 promotes translation in human cancer cells (2016, methodology): “Reads mapping, m6A peak calling, and motif search were performed as described (Dominissini et al., 2013).”
- Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA (2016, methodology): “To measure m(6)A NANOGmRNA levels, m(6)A immunoprecipitation was performed as described before (47).”
- N 6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein (2017, methodology): “The PAR-CLIP–methylated RNA immunoprecipitation (MeRIP) experiment applied m6A-antibody immunoprecipitation (31) to the HNRNPG PAR-CLIP RNA samples (from HEK293T cells in eight 15-cm plates).”
- The potential role of RNA N6-methyladenosine in Cancer progression (2020, background): “Because the small RNA fragment targeted by MeRIP-m6A-seq has a resolution of approximately 100 nt, single-nucleotide variations at the methylated site cannot be accurately detected [7, 58].”