High-Throughput Methods for Culturing Microorganisms in Very-Low-Nutrient Media Yield Diverse New Marine Isolates

By S. Connon, S. Giovannoni. Applied and Environmental Microbiology, 2002

769 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/a513118de289d3df1aed57ad0feca2237d4690aa

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Citations in the corpus

Strategies for culture of 'unculturable' bacteria. (2010, background): “…(Hahn et al., 2004), density-gradient centrifugation or elutriation and extinction-dilution whereby samples are diluted, ideally down to single cells, before their culture in isolation (Watve et al., 2000; Connon & Giovannoni, 2002; Ben-Dov et al., 2009; Song et al., 2009; Wang et al., 2009).”
Oligotyping: Differentiating between closely related microbial taxa using 16S rRNA gene data (2013, background): “Only few isolates are available due to the challenging cultivation procedures (Connon & Giovannoni 2002; Rappe et al. 2002; Stingl, Tripp & Giovannoni 2007; Carini et al. 2013); hence, the depth of taxonomic classification for proper identification of different SAR11 organisms in environmental…”
Growing Unculturable Bacteria (2012, background): “This culture technique has now led to the culture of new members of this clade from different marine regions (75) as well as other previously uncultured isolates from both marine and freshwater environments (15, 33, 36, 59, 79).”
Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial Species (2010, background): “The renewed interest in microbial cultivation (1, 5, 7, 8, 14, 15, 33, 42) has led to the development of several innovative approaches to bring new species into culture.”
Culturing the marine cyanobacterium Prochlorococcus (2007, methodology): “Other methods of isolation, such as the high-throughput methods introduced by Connon and Giovannoni (2002), should be explored to isolate potentially novel Prochlorococcus.”
Recent progress in biohydrometallurgy and microbial characterisation (2018, methodology): “This method can be laborious and time-consuming and is most useful for samples with relatively low microbial diversity (Connon and Giovanni, 2002; Stewart, 2012).”
Metagenomics: Application of Genomics to Uncultured Microorganisms (2004, background): “(34, 38, 113), which represents more than one-third of the”
The Selective Value of Bacterial Shape (2006, background): “This is very close to the size of the smallest bacteria observed in oligotrophic oceanic environments, these cells being tiny rods or coccoidal cells from 300 to 500 nm in diameter (44, 227, 266).”
Cleaning up with genomics: applying molecular biology to bioremediation (2003, background): “This search for previously uncultured organisms can be greatly accelerated with high-throughput culturing and screening strategie”