Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited

By Brian P. English, Wei Min, A. V. van Oijen, Kang Taek Lee, G. Luo, Hongye Sun, B. Cherayil, Samuel C. Kou, Xiaohui S. Xie. Nature Chemical Biology, 2006

720 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a50dab835002134d30359a1d7cdd3a00c0352439

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Atomic and Molecular Physics, and Optics

1. Frank No'e
2. Hao Wu
3. Christof Schutte
4. Ting Lu
5. Xiaona Fang
6. Karsten Kruse
7. Jin Wang
8. John D. Chodera

In Biophysics

1. Oren Z. Kraus
2. Brendan J. Frey

In Molecular Biology

1. Frank No'e
2. Hao Wu
3. Christof Schutte
4. Cecilia Clementi
5. Ting Lu
6. Xiaona Fang
7. Karsten Kruse
8. John D. Chodera

Citations in the corpus

• Cellular heterogeneity: when do differences make a difference? (2010, background): “Although we have focused our discussion on cells, heterogeneity—and the loss of information due to ensemble averages—has been studied at many scales of biology, from single molecules (English et al., 2006) to communities of whole populations (Kimura and Weiss, 1964).”
• Real-time DNA sequencing from single polymerase molecules. (2010, background): “While the validity of the Michaelis–Menten equation has been demonstrated theoretically and experimentally in the presence of such dynamic variation (English et al., 2006; Kou et al., 2005; Min et al., 2006; Velonia et al., 2005), the extracted parameters do not inform about it, so a more detailed a”
• Real-Time DNA Sequencing from Single Polymerase Molecules (2009, background): “While the validity of the Michaelis–Menten equation has been demonstrated theoretically and experimentally in the presence of such dynamic variation (English et al., 2006; Kou et al., 2005; Min et al., 2006; Velonia et al., 2005), the extracted parameters do not inform about it, so a more detailed a”
• Nonequilibrium physics in biology (2019, background): “Following Michaelis and Menten, the rate of the kinetics is obtained under the assumption that the substrate and the substrate-enzyme complex are in equilibrium (English et al., 2006; Michaelis and Menten, 1913; Xie, 2013).”
• Transient dynamics of terrestrial carbon storage: mathematical foundation and its applications (2017, background): “Oscillations have been documented for single enzymes at timescales between 10−4 and 10 s (English et al., 2006; Goldbeter, 2013; Xie, 2013).”
• Markov models of molecular kinetics: generation and validation. (2011, background): “Enzyme kinetics has been shown to be modulated by interchanging conformational substates [14].”