Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP.

By G. Feng, Rebecca Mellor, M. Bernstein, Cynthia R. Keller-Peck, Q. Nguyen, Mia Wallace, J. Nerbonne, Jeff William Lichtman, J. Sanes. Neuron, 2000

3,123 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a421c0fff0daf9f527cd0fd59938699c2d6b7690

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Citations in the corpus

Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain (2011, neutral): “To characterize the expansion of macroscopic structures in two dimensions, we time-lapse imaged fixed brain slices (1 mm thick) of a transgenic mouse line, thy1-YFP line H (YFP-H”
Resting Microglia Directly Monitor the Functional State of Synapses In Vivo and Determine the Fate of Ischemic Terminals (2009, methodology): “To investigate possible contacts between microglial processes and neuronal elements of brain circuits, we crossbred the Iba1–EGFP mouse with a Thy1 promoter GFP mouse (M-line) (Feng et al., 2000) and used line scanning to examine possible contacts between microglia and neuronal structures, and we fo”
Massive restructuring of neuronal circuits during functional reorganization of adult visual cortex (2008, background): “in the monocular visual cortex of mice expressing green fluorescent protein (GFP) in a subset of neurons (Thy1 GFP–M line”
Cell type-specific structural plasticity of axonal branches and boutons in the adult neocortex. (2006, positive): “Transgenic mice expressing membrane-bound GFP (line 15; De Paola et al., 2003) and cytoplasmic GFP (line GFP-M; Feng et al., 2000 ) had sparsely labeled neurons in the thalamus and L2/3, 5, and 6 in the neocortex (Figure 1 and see Figure S1 in the Supplemental Data available online).”
Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window (2009, neutral): “(5) Similarly, big stubby spines can disappear, but the dendritic shaft retains increased curvature at the site (Fig.”
iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. (2014, positive): “Using an Abcam anti-GFP antibody (Table S3), we tested iDISCO on the Thy1::GFP-M line, which has the most sparse GFP expression of all reported Thy1 lines (Feng et al., 2000).”
A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging (2016, background): “In mice expressing YFP in a sparse subset of pyramidal neurons (YFP-H) (Feng et al. 2000) we were able to resolve individual spines (Figure 7 Supplement 3).”
Locally dynamic synaptic learning rules in pyramidal neuron dendrites (2007, positive): “Acute hippocampal brain slices were prepared from Thy1 GFP mice 19 (line M; postnatal day 14–18).”
Deep tissue two-photon microscopy (2005, background): “Widespread but cell-specific labeling can be achieved in transgenic animals expressing anatomical marker”