High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota

By Erwin G. Zoetendal, M. Rajilić-Stojanović, Willem M. de Vos. Gut, 2008

696 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/a23f5efc837a6873ba0f737812ef923ccab6bceb

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Surgery

Citations in the corpus

Intestinal microbiota in functional bowel disorders: a Rome foundation report (2012, background): “High-throughput DNA sequencing provides completely new possibilities for ‘-omics’-based analyses of the gut microbiota.(19) Draft genomes of cultured gut bacteria can now be produced rapidly and at little cost.”
Microbial Dysbiosis in Colorectal Cancer (CRC) Patients (2011, background): “gut immune function, and protection against pathogens and diseases [2–5].”
The human small intestinal microbiota is driven by rapid uptake and conversion of simple carbohydrates (2012, background): “The GI tract is colonized with approximately 1000 microbial species, commonly called the microbiota, and may harbor more than nine million unique genes (Zoetendal et al., 2008; Yang et al., 2009).”
Diversity of Bifidobacteria within the Infant Gut Microbiota (2012, methodology): “Nevertheless, even if the DNA extraction and PCR amplification protocols, as presented in this study, demonstrated good potential for bifidobacterial profiling of human gut ecosystems, these still come with some limitations, such as the potential effects on amplification yields due to increased size”
Composition, variability, and temporal stability of the intestinal microbiota of the elderly (2010, background): “R advances in culture-independent techniques for microbial community analysis (1, 2) have led to major progress in understanding the complexity of the human intestinal ecosystem (reviewed in ref.”
Microbes inside—from diversity to function: the case of Akkermansia (2012, background): “…on phylogenetic microarrays or parallel sequencing of barcoded small subunit rRNA amplicons, in combination with improved bioinformatics tools, have allowed the large-scale description of microbial communities in the human gut (Sogin et al., 2006; Zoetendal et al., 2008; Schloss et al., 2009).”
Through Ageing, and Beyond: Gut Microbiota and Inflammatory Status in Seniors and Centenarians (2010, background): “The majority of earlier studies has relied on cultivation-based approaches but high-throughput culture-independent molecular techniques are considered essential to provide a global insight in the intestinal microbiota [19].”
Intestinal microbiota in human health and disease: the impact of probiotics (2011, background): “In addition to the two phyla Bacteroidetes and Firmicutes, also members of other phyla, such as Proteobacteria, Actinobacteria, Fusobacteria, Spirochaetes, Verrucomicrobia and Lentisphaerae, have been detected (Rajilić-Stojanović et al. 2007; Zoetendal et al. 2008).”
The first 1000 cultured species of the human gastrointestinal microbiota (2014, methodology): “However, the complexity of the gastrointestinal tract microbial ecosystem hampers the rapid application of SSU rRNA-based methods as well as (meta)genomics (Zoetendal et al., 2008).”
Comparative Analysis of Pyrosequencing and a Phylogenetic Microarray for Exploring Microbial Community Structures in the Human Distal Intestine (2009, positive): “…been supported by quantitative analysis of the ubiquitous microbial 16S ribosomal RNA gene using traditional molecular methods like denaturing gradient gel electrophoresis (DGGE), fluorescent in situ hybridization (FISH), quantitative PCR (qPCR), or capillary sequencing using the Sanger method [7].”
Emerging molecular insights into the interaction between probiotics and the host intestinal mucosa (2011, background): “In particular, the rapidly advancing research into the potentially beneficial effects of specific microbial species in the human intestinal microbiota, in circumstances of health and diseas”